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| Status |
Public on Aug 30, 2008 |
| Title |
Activity Deprivation-Induced Transcriptional Changes in the P21 Cochlear Nucleus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We analyzed whether cochlear removal-induced transcriptional changes in the cochlear nucleus (CN) were due to loss of electrical activity in the 8th nerve. Pharmacological activity blockade of the auditory nerve for 24 h resulted in similar expression changes for only a subset of genes. Thus, an additional factor not dependent on action potential-mediated signaling must also regulate transcriptional responses to deafferentation in the CN.
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| Overall design |
TTX was infused into the cochlea unilaterally via osmotic pumps to block activity in the 8th nerve. CN tissue was dissected out from 10-12 mice per replicate and pooled. Three biological replicates were performed. Gene expression was compared between the ipsilateral and contralateral CN tissue to identify genes transcriptionally regulated after activity deprivation.
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| Contributor(s) |
Harris JA, Rubel EW |
| Citation(s) |
18945907 |
| |
| Submission date |
Jun 09, 2008 |
| Last update date |
May 04, 2018 |
| Contact name |
Julie Harris |
| E-mail(s) |
jharris@gladstone.ucsf.edu, rubel@u.washington.edu
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| Organization name |
University of Washington
|
| Department |
Otolaryngology-HNS
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| Lab |
Ed Rubel
|
| Street address |
UW Box 357923
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115 |
| Country |
USA |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA106027 |
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