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| Status |
Public on May 19, 2018 |
| Title |
An essential role for Abscisic acid in the regulation of xylem fibre differentiation |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The Arabidopsis hypocotyl is an excellent model for understanding radial growth in plants. Division of the cambial cells and their subsequent differentiation into xylem and phloem drives radial expansion of the hypocotyl. Following the transition to reproductive growth, a phase change occurs in the Arabidopsis hypocotyl. During this second phase, the relative rate of xylem production is dramatically increased compared to that of phloem and xylem fibres containing thick secondary cell walls also form, which results in the production of xylem tissue comparable to the wood of trees. Abscisic acid (ABA) is a phytohormone known to have a major role in various plant processes, including in the response to changes in environmental conditions and in the promotion of seed dormancy. Using two different genetic backgrounds and different environmental conditions, we identified a set of core of transcriptional changes associated with the switch to the second phase of growth in the hypocotyl. ABA signalling pathways were identified as being as significantly over-represented in this set of core genes. Reverse genetic analysis demonstrated that mutants defective in ABA-biosynthesis enzymes exhibited significantly delayed fibre production without affecting the xylem:phloem ratio. The altered morphology is also reflected at the transcript level, with a reduced expression of marker genes associated with fibre formation in aba1 mutants. The application of exogenous ABA to the mutant rescued the phenotype, restoring fibre differentiation to wild-type levels. Taken together the data reveals an essential role for ABA in the regulation of fibre formation.
We used microarrays to probe transcripome changes I Arabidopsis hypocotyls following transition from phase I to phase II
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| Overall design |
Arabidopsis thaliana, Col-0 plants were grown under short day conditions for 6 weeks and switched to long days to induce flowering. RNA was extracted in triplicates from the hypocotyls of plants 0, 1, 2, 4 and 10 days following the day length switch, with 8 individual plants pooled per replicate
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| Contributor(s) |
Kumar M, Campbell L, Etchells JP, Cooper M, Turner SR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 18, 2018 |
| Last update date |
May 20, 2018 |
| Contact name |
Manoj Kumar |
| E-mail(s) |
manoj.kumar@manchester.ac.uk
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| Phone |
01612751710
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| Organization name |
University of Manchester
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| Department |
Faculty of Biology, Medicine and Health
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| Lab |
Simon Turner
|
| Street address |
Oxford Road
|
| City |
Manchester |
| State/province |
Lancashire |
| ZIP/Postal code |
M13 9PT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL17416 |
[AraGene-1_0-st] Arabidopsis Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (15)
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| GSM3146319 |
0 days after day length switch - replicate 1 |
| GSM3146320 |
0 days after day length switch - replicate 2 |
| GSM3146321 |
0 days after day length switch - replicate 3 |
| GSM3146322 |
1 days after day length switch - replicate 1 |
| GSM3146323 |
1 days after day length switch - replicate 2 |
| GSM3146324 |
1 days after day length switch - replicate 3 |
| GSM3146325 |
2 days after day length switch - replicate 1 |
| GSM3146326 |
2 days after day length switch - replicate 2 |
| GSM3146327 |
2 days after day length switch - replicate 3 |
| GSM3146328 |
4 days after day length switch - replicate 1 |
| GSM3146329 |
4 days after day length switch - replicate 2 |
| GSM3146330 |
4 days after day length switch - replicate 3 |
| GSM3146331 |
10 days after day length switch - replicate 1 |
| GSM3146332 |
10 days after day length switch - replicate 2 |
| GSM3146333 |
10 days after day length switch - replicate 3 |
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| Relations |
| BioProject |
PRJNA472031 |
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