|
| Status |
Public on May 02, 2019 |
| Title |
RNA-Seq of isogenic human iPS cell-derived cardiomyocytes with RBM20 mutations created by genome editing [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RBM20 heterozygous (Het) and homozygous (Homo) R636S and homozygous 8-bp deletion (which causes nonsense-mediated decay) mutations were created by genome editing in an iPS cell line generated from a healthy male patient. Those cells were differentiated into cardiomyocytes by modulating the WNT signaling pathway, and then are subjected to the lactate purification method.
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| |
|
| Overall design |
RNAseq of iPS cell-derived cardiomyocytes with 4 different genotypes as replicates
|
| |
|
| Contributor(s) |
Miyaoka Y, Mayerl SJ, Chan AH, Conklin BR, Salomonis N |
| Citation(s) |
34732726 |
| |
| Submission date |
May 02, 2018 |
| Last update date |
Dec 02, 2021 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (21)
|
| GSM3124390 |
WTC11 RBM20 8-bp deletion Homo replicate 1 |
| GSM3124391 |
WTC11 RBM20 8-bp deletion Homo replicate 2 |
| GSM3124392 |
WTC11 RBM20 8-bp deletion Homo replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE175886 |
RNA-Seq of isogenic human iPS cell-derived cardiomyocytes with RBM20 mutations created by genome editing |
|
| Relations |
| BioProject |
PRJNA454577 |
| SRA |
SRP144316 |
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