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| Status |
Public on Jun 30, 2018 |
| Title |
Development of gene expression signatures for lenalidomide treatement on megakaryocyte |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To clarify the machinery how lenalidomide inhibit proplatelet formation of megakaryocytes, we performed transcriptome analysis of megakaryocytes derived from human hematopoietic stem/progenitor cells in the liquid culture system with or without lenalidomide. Differentially expressed genes were comprehensively evaluated by gene set enrichment analysis. Untreated megakaryocytes showed the estradiol-responded gene expression signatures, whereas the lenalidomide-treated megakaryocytes did not. Furthermore, we performed a rescue strategy with exogenous estradiol, confirming that lenalidomide-induced inhibition of proplatelet formation should be due to the deficiency of endogenous estradiol action in megakaryocytes.
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| Overall design |
Lenalidomide treatment-induced difference in gene expression profile of human megakaryocytes were evaluated. Megakaryocytes were generated from hematopoietic stem/progenitor cells of 4 individual healthy volunteers in the presence or absence of 10 µM lenalidomide.
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| Contributor(s) |
Tochigi T, Kikushige Y, Miyamoto T, Hatakeyama K, Sakoda T, Kato K, Takenaka K, Maeda T, Ito T, Handa H, Akashi K |
| Citation missing |
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| |
| Submission date |
Mar 19, 2018 |
| Last update date |
Jul 01, 2018 |
| Contact name |
Taro Tochigi |
| E-mail(s) |
taro_tochigi@yahoo.co.jp
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| Organization name |
Kyushu university
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| Street address |
Maidashi
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| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA438898 |
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