GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE110852 Query DataSets for GSE110852
Status Public on Dec 26, 2019
Title Gene expression profiles of subjects exposed to Arsenic through drinking water in Pakistan
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Ground water Arsenic toxicity is the global problem and millions of people are exposed to elevated levels through drinking water than WHO permiscible limit of 10µg/L. The exposure is associated with various cancerous and non-cancerous diseases. It may alter the gene expression profile of invidual and suppress the activity of various genes giving rise to different diseases. Pakistan, a developing country in South Asian region, also have different areas reported to have elevated Ground water As. levels since 2005. The Present Study aimed to investigate Transcriptome profile of the exposed subjects in two districts of Punjab Province Pakistan. i.e Lahore and Kasur. The population was stratified into three exposure groups comprising Low, Medium and High exposure based on their urinary arsenic levels. We used Agilent Microarrays platform for Transcriptomics analysis and Linear mixed model was used to find differential gene expression associated with Arsenic exposure
Overall design Whole blood samples stored in RNA later were collected form the respected exposure groups and transported to Department of Toxicogenomics Maastricht University, The Netherlands for further analysis. Total RNA was Isolated using Ribopure Blood RNA kit. Agilent one colour Microarrays protocol was used for Labeling, Hybridization and scanning.
Contributor(s) Rehman MY, Kleinjans JC, Briede JJ, van Herwijnen M, Krauskopf J, Jennen D, Farooqi A, Malik RN
Citation(s) 31864032
Submission date Feb 20, 2018
Last update date Dec 26, 2019
Contact name Muhammad Yasir Abdur Rehman
Phone +923515357447
Organization name Quaid-i-Azam University
Department Environmental Sciences
Lab Environmental Health Laboratory
Street address 1
City Islamabad
State/province ICT
ZIP/Postal code 45320
Country Pakistan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (57)
GSM3017820 SM16
GSM3017821 SM18
GSM3017822 SM19
BioProject PRJNA434684

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110852_RAW.tar 703.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap