Human T cell clones were derived according to standard protocols, once assessed for their functional profile were studied for gene expression. The purpose of this study is to analyze human classic Th1, non-classic Th1 and Th17 clones with and without stimulation with anti-CD2+anti-CD3+anti-CD28 coated beads to measure differences in gene expression.
Overall design
5 different T helper cell clones were analyzed for each phenotype. Each clone was analyzed either resting either after 6 hours stimulation with anti-CD2+anti-CD3+anti-CD28.