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| Status |
Public on Sep 01, 2018 |
| Title |
Transcriptome analysis of wild-type and maternal ybx1 mutant zebrafish embryos during early development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To study the function of zebrafish Ybx1 during early embryogenesis, we generated maternal ybx1 (Mybx1) mutant using CRISPR/Cas9 and report the transcriptome-wide changes in comparison to wild-type (WT) embryos. Our analysis reveals a dramatic loss of maternal mRNA decay and zygotic genome activation in Mybx1 embryos during maternal-to-zygotic transition.
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| Overall design |
Total RNA samples were prepared using WT and Mybx1 embryos at 0 hpf and 6 hpf. Ribosomal RNA depletion was performed before RNA-seq.
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| Contributor(s) |
Sun J, Meng A |
| Citation(s) |
30135188 |
| |
| Submission date |
Jan 09, 2018 |
| Last update date |
Nov 04, 2018 |
| Contact name |
Jiawei Sun |
| E-mail(s) |
jiawei.sun@hotmail.com
|
| Organization name |
School of Life Sciences, Tsinghua University
|
| Lab |
Anming Meng Lab
|
| Street address |
Medical Science Building, Tsinghua University
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA429172 |
| SRA |
SRP128504 |
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