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| Status |
Public on Sep 30, 2020 |
| Title |
Gene expression of human hematopoietic stem cells (CD34+) stimulated with native & mutant (244) form of myeloid stem-cell transcription factor PU.1 alone & in combination with myeloid specific cytokines (IL3+GMCSF+Flt2+MCSF+SCF) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human CD34+ hematopoietic stem cells gives rise to complete blood lineages through the process of hematopoiesis. Here we tried to differentiate a particular lineage i.e myeloid from CD34+ hematopoietic stem cells using a transcription factor PU.1 and its mutated form. Gene expression analysis was performed after treating cells with protein factors and different cytokine combinations. Cells were given six different treatments including control-PU.1 only and PU.1 with cytokines combination; Y244D only and Y244D with cytokines combination and only cytokine combination (IL3, GMCSF, Flt2, MCSF, and SCF). After 5 days of incubation, cells were washed and pellet was preserved in RNAlater (Ambion) to be shipped to Genotypic Technologies Pvt. Ltd. (Bangalore) for microarray analysis.
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| Overall design |
HSC Cells were given six different treatments including control-PU.1 only and PU.1 with cytokines combination; Y244D only and Y244D with cytokines combination and only cytokine combination (IL3, GMCSF, Flt2, MCSF, and SCF). After 5 days of incubation, cells were washed and pellet was preserved in RNAlater (Ambion) to be shipped to Genotypic Technologies Pvt. Ltd. (Bangalore) for microarray analysis.
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| Contributor(s) |
Gangenahalli G, Joshi M, Gupta P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 03, 2018 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Gurudutta U Gangenahalli |
| E-mail(s) |
gugdutta@rediffmail.com
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| Phone |
9101123905144
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| Organization name |
Institute of Nuclear Medicine & Allied Sciences
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| Department |
Stem Cell Research Division
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| Lab |
Stem Cell & Gene Therapy Research Group
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| Street address |
Brig. S. K. Mazumdar Marg, Timarpur
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| City |
Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110054 |
| Country |
India |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (18)
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| GSM2912509 |
control hematopoetic stem cells replicate 1 |
| GSM2912510 |
control hematopoetic stem cells replicate 2 |
| GSM2912511 |
control hematopoetic stem cells replicate 3 |
| GSM2912512 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF replicate 1 |
| GSM2912513 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF replicate 2 |
| GSM2912514 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF replicate 3 |
| GSM2912515 |
HSC treated with PU-1 protein replicate 1 |
| GSM2912516 |
HSC treated with PU-1 protein replicate 2 |
| GSM2912517 |
HSC treated with PU-1 protein replicate 3 |
| GSM2912518 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF,PU-1 protein replicate 1 |
| GSM2912519 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF,PU-1 protein replicate 2 |
| GSM2912520 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF,PU-1 protein replicate 3 |
| GSM2912521 |
HSC treated with PU-1 Y244D mutant protein replicate 1 |
| GSM2912522 |
HSC treated with PU-1 Y244D mutant protein replicate 2 |
| GSM2912523 |
HSC treated with PU-1 Y244D mutant protein replicate 3 |
| GSM2912524 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF,PU-1 Y244D protein replicate 1 |
| GSM2912525 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF,PU-1 Y244D protein replicate 2 |
| GSM2912526 |
HSC treated with IL-3, SCF, FLT-3,GMCSF,MCSF,PU-1 Y244D protein replicate 3 |
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| Relations |
| BioProject |
PRJNA428367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE108720_RAW.tar |
213.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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