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| Status |
Public on Dec 20, 2017 |
| Title |
Transcriptional regulation of adaptive NK and CD8 T cell responses [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Natural killer (NK) cells are innate lymphocytes that possess features of adaptive immunity such as clonal expansion and generation of long-lived memory. Here we look at transcriptional profiles of NK cells throughout several time points during MCMV infection, ex-vivo cytokine stimulation, and/or deficiency of key transcription factors such as STAT4, STAT1, and Runx1. In addition, we profile parallel time points of MCMV-specific CD8 T cells during infection and memory formation.
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| Overall design |
RNA-seq was performed on ex-vivo NK cultured overnight in cytokines or NK cells and CD8 T cells harvested during in-vivo infection. Cytokine conditions are as follows: unstim, IL-12 (20 ng/ml), IL-12 (20 ng/ml) and IL-18 (10 ng/ml), IFNa (100 U/ml) stim, IL-15 (10 ng/ml).
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| Contributor(s) |
Lau CM, Sun JC |
| Citation(s) |
29222089, 30134157, 30082830, 32045471 |
| |
| Submission date |
Oct 25, 2017 |
| Last update date |
May 04, 2023 |
| Contact name |
Colleen Lau |
| Organization name |
Cornell University
|
| Department |
Microbiology & Immunology
|
| Street address |
930 Campus Road
|
| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14850 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (88)
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| This SubSeries is part of SuperSeries: |
| GSE106139 |
Regulation of natural killer cells during MCMV infection |
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| Relations |
| BioProject |
PRJNA415721 |
| SRA |
SRP121410 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106138_ExVivoCytokineStim_unstimIFNaonly_rawCounts_knownGene.txt.gz |
776.2 Kb |
(ftp)(http) |
TXT |
| GSE106138_ExVivoCytokineStim_unstimIL1218IL15only_rawCounts_knownGene.txt.gz |
841.4 Kb |
(ftp)(http) |
TXT |
| GSE106138_ExVivoCytokineStim_unstimIL12only_rawCounts_knownGene.txt.gz |
770.7 Kb |
(ftp)(http) |
TXT |
| GSE106138_IFNARKOChimera_MCMV_IfnarHpos_rawCounts_knownGene.txt.gz |
880.8 Kb |
(ftp)(http) |
TXT |
| GSE106138_IL12RKOChimera_MCMV_IL12rHpos_rawCounts_knownGene.txt.gz |
971.4 Kb |
(ftp)(http) |
TXT |
| GSE106138_MCMV_RNA_NKCD8m45_rawCounts_knownGene.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE106138_MCMV_RNA_d0d2Only_rawCounts_knownGene.txt.gz |
787.1 Kb |
(ftp)(http) |
TXT |
| GSE106138_RNAseq_Irf9KO_MCMV_rawCounts.txt.gz |
805.4 Kb |
(ftp)(http) |
TXT |
| GSE106138_RUNX1KOChimera_MCMV_F1M1M2_rawCounts_knownGene.txt.gz |
848.9 Kb |
(ftp)(http) |
TXT |
| GSE106138_STAT1KOChimera_MCMV_Stat1Hpos_rawCounts_knownGene.txt.gz |
911.1 Kb |
(ftp)(http) |
TXT |
| GSE106138_STAT4KOChimera_MCMV_Stat4HposHneg_rawCounts_knownGene.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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