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| Status |
Public on Sep 15, 2017 |
| Title |
Microprocessor recruitment to elongating RNA Polymerase II is required for differential expression of microRNAs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The cellular abundance of mature microRNAs (miRNAs) is dictated by the efficiency of nuclear processing of primary miRNA transcripts (pri-miRNAs) into pre-miRNA intermediates. The Microprocessor complex of Drosha and DGCR8 carries this out, but it has been unclear what controls Microprocessor's differential processing of various pri-miRNAs. Here, we show that Drosophila DGCR8 (Pasha) directly associates with the C terminal domain of the RNA polymerase II elongation complex when it is phosphorylated by the Cdk9 kinase (pTEFb). When association is blocked by loss of Cdk9 activity, a global change in pri-miRNA processing is detected. Processing of pri-miRNAs with a UGU sequence motif in their apical junction domain increases, while processing of pri-miRNAs lacking this motif decreases. Therefore, phosphorylation of RNA polymerase II recruits Microprocessor for co-transcriptional processing of non-UGU pri-miRNAs that would otherwise be poorly processed. In contrast, UGU-positive pri-miRNAs are robustly processed by Microprocessor independent of RNA polymerase association.
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| Overall design |
There are 2 samples, one control and one experimental
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| Contributor(s) |
Church VA, Carthew RW |
| Citation(s) |
28954229 |
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| Submission date |
Aug 29, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Carthew |
| Organization name |
Northwestern University
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| Street address |
2205 Tech Drive
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| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
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| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA400598 |
| SRA |
SRP116390 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103234_CARTHEW_PROCESSED_miRNA_Reads.xlsx |
53.0 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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