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Series GSE102183 Query DataSets for GSE102183
Status Public on Aug 23, 2017
Title Targeting the androgen receptor N-terminus via the cochaperone Bag-1L [RNA-seq C-terminal mutant]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Targeting the activation function-1 (AF-1) at the N-terminus of the androgen receptor (AR) is an attractive therapeutic alternative to the current approaches to inhibit AR action in prostate cancer (PCa). Here we show that the AR AF-1 is bound by the BAG domain of the cochaperone Bag-1L. Mutations in this domain or loss of Bag-1L abrogates AR signaling and reduces PCa growth. Correspondingly, Bag-1L protein levels increase with progression of primary prostate tumors to castration-resistant PCa, correlating inversely with patient response to abiraterone therapy. Intriguingly, BAG domain residues important for its interaction with the AR AF-1 overlap a potentially druggable pocket of this protein. Bag-1L is therefore a putative therapeutic target for the inhibition of AR AF-1 activity.
Overall design We performed transcriptome analysis (RNA-Seq) of 2 cell lines (LNCaP Bag-1L WT and LNCaP Bag-1L CMut) cultured under hormone-starvation conditions for 72 h and then treated with vehicle (ethanol, EtOH) or 10 nM Dihydrotestosterone (DHT) for 16 h. Biological duplicate samples were analyzed for each cell line and each treatment condition.
Contributor(s) Cato L, Neeb A, Armant O, Gourain V, Cato AC, Brown M
Citation(s) 28826504, 35479411
Submission date Aug 02, 2017
Last update date May 05, 2022
Contact name Laura Cato
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 450 Brookline Ave., D728
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (8)
GSM2730045 LNCaP Bag-1L WT ETOH rep1
GSM2730046 LNCaP Bag-1L WT ETOH rep2
GSM2730047 LNCaP Bag-1L WT DHT rep1
This SubSeries is part of SuperSeries:
GSE89939 Targeting the androgen receptor N-terminus via the cochaperone Bag-1L
BioProject PRJNA396941
SRA SRP114702

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102183_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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