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Platform GPL8513 Query DataSets for GPL8513
Status Public on May 03, 2009
Title Agilent-018073 Custom Brassica 60-mer two-color microarray
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Brassica
Manufacturer Agilent Technologies
Manufacture protocol The assembled Brassica sequences (94,558 instances including those represented in both orientations) were submitted to Agilent Technologies’ eArray web portal for gene expression probe design. Probes with a base composition score greater than or equal to 3 were omitted from the final design; resulting in a total of 91,854 unique probes (including 6,989 derived from oppositely oriented pairs of sequences) that were included in the microarray design, of which 10,466 were predicted to have cross-hybridization potential.
11,893 probes were randomly selected to be represented in duplicate in the final design, which also included Agilent Technologies’ standard panel of quality control and spike-in probes.
Microarrays were manufactured using Agilent Technologies’ SurePrint Technology in the 2x104k format (two arrays containing ~104,000 probes on a single 1” x 3” glass slide).
 
 
Submission date Apr 30, 2009
Last update date May 08, 2015
Contact name Andreas Magusin
E-mail(s) andreas.magusin@jic.ac.uk
Organization name John Innes Centre
Department Crop Genetics
Street address Norwich Research Park
City Norwich
ZIP/Postal code NR47UH
Country United Kingdom
 
Samples (203) GSM399515, GSM399516, GSM399517, GSM399518, GSM399519, GSM399520 
Series (6)
GSE15915 Transcription profiling of Brassica lines
GSE23141 Evaluation of the Affymetrix Brassica Exon 1.0 ST array
GSE23409 Comparison of root and leaf samples from Brassica rapa
Relations
Alternative to GPL20168

Data table header descriptions
ID
GB_LIST gb accession
PT_ACC protien accession
SEQUENCE sequence
ProbeName probe id
GB_ACC gb accession
GeneName gene name
Description description
SPOT_ID spot identifier

Data table
ID GB_LIST PT_ACC SEQUENCE ProbeName GB_ACC GeneName Description SPOT_ID
1 GE_BrightCorner GE_BrightCorner
2 DarkCorner DarkCorner
3 DarkCorner DarkCorner
4 Q9M0P0 GTCTCGTCATGTTCCTGACTTCTCATGACAAGTAACACATATATCTGATGTGTTATAATT B_1007874 DY023795 Putative uncharacterized protein AT4g09530
5 GCGGAACTCGGCTGCTAGTTTGCTTTTCGTTAAAAGACAAATATAATATATATGCAATGT X_1000035 AI352956 Unknown
6 EE448061, EE502843, EE502716 Q94KI8 GTGTTCTACTCCTGATACGTAATATAGCTCGTAAAAAGGTCAAAATCTTGATTCTACTGA B_1046053 JCVI_10279 Putative calcium channel
7 Q9FKP2 TCGTCGATTACCTCTCCTTACTCTACTGCAACTTCGAAAAGTTTCCACTTTTGGGCCAAT B_1002675 CD824703 Na/Ca,K-exchanger-like protein
8 EE529079, CD827748 AAGGTTTGATCCTTGGGTGTTATTGTAGGTTGCTATCCTTGTAACACATGTGGATTTCAT B_1078619 JCVI_39689 Unknown
9 CTAAGGAGCCGCAAAAAGTTTTTAAAAACCCTTAAGAACCTAGATTATTAAGTCCAAGCT B_1037671 EV192881 Unknown
10 ES936586, EV005214, ES951475, EE482968, ES919717, EV017382, ES951125, CN737138, EE540372, EX133740 CGGTGTAGTTTCTACCCTTCTACTTATCGTAAGTGGAATTTTCTTTTTTCTGATTGCAGG B_1055431 JCVI_18733 At3g01435
11 EX091920, CX190799, CV546606, EV018322, EX101084 Q8H0Z9 GACTTTGGAATCCGAACGCGTAAAAGCATCCATCAGACCAAAACTTATGCTTTATTTTTT B_1075533 JCVI_3689 Putative uncharacterized protein At5g13090
12 EX136730, ES911756, EX122260, EE567568 Q39254 CAAAATGTTTCTACTACACTGCGCTGCCTTCTCCTGTGGGGTGGTATTGTATCACAAAAA B_1055245 JCVI_18566 Vacuolar cation/proton exchanger 2 (Ca(2+)/H(+) exchanger 2) (Ca(2+)/H(+) antiporter CAX2)
13 O80845 CTAGGTTTCTTGTTTGAACTAATGTTCTTGGTTTATATCATTACAGAACAAAGAGCGAGC B_1023804 ES968722 Expressed protein
14 ES907662, DY003176, EX098251, EE507592, EE548921, EE552353, EE544883, EV084713, EE523032 Q6DBN8 GTTGTTGCTGTATATTCTCTCTGACTTCTCTTATTCAACAAGGAAGGTTTTGAGTAATCT B_1056762 JCVI_19932 At5g23610
15 Q0WKV7 GCAACACAGCGTGAGTTTGAGTTGGAAACGGAGCAGAAAAGAAAATCTATTGATGACAGC B_1027259 EV060006 Putative uncharacterized protein At1g67230
16 EV039854, AM389964, CX281097, DN192321, AM395027 A2RVQ5 TATTTTAAGTTTCCTCTGCAAAGAACATTGGGTTTGGCGTTTATGCATTGTTCTGGTGAT B_1059715 JCVI_22601 At3g57230
17 CTTCCAGATCAATGAATTAAAACAAACGAAACTAAATCTGAGTGAGAGACATACCTTTCG B_1023996 ES976722 Unknown
18 EX088096, EE400793, ES912659, EE477375, ES948118, EV187556, EE563643 Q9CAN4 GTTGAGGCGGTTTTAAAAGTCTTAAAACCGAAGTTTGGTTTGGTTGTAAAAGCTATACTT B_1072711 JCVI_34337 F-box protein PP2-A11
19 EX080945, EX080684 Q9RGU1 GAAGCAGCGTCCAAGTAAGACTCTCCTCCTCCATAGATCCTTTATTGTTTTTGCTTAAGA B_1061337 JCVI_24070 Putative uncharacterized protein
20 A5B4P5 GAGAGCTGCGGAAGTCGCTGGAGGATCAGGGTAATCAAAAACTTGTACATTTGGAAAGAT X_1008694 EE411718 Putative uncharacterized protein

Total number of rows: 105072

Table truncated, full table size 14823 Kbytes.




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