GEO DataSets

(Submitter supplied) We propose a strain engineering approach for synthesizing sedoheptulose using glucose as sole feedstock. The gene pfkA encoding 6-phosphofructokinase in Corynebacterium glutamicum was inactivated to direct the carbon flux towards pentose phosphate pathway in the cellular metabolic network. This genetic modification successfully enabled the synthesis of sedoheptulose from glucose. Additionally, we identified key enzymes responsible for product formation through transcriptome analysis, and their corresponding genes were overexpressed, resulting in a further 20% increase in sedoheptulose production.

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34144

4 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL32785

12 Samples

Download data: TXT

(Submitter supplied) mRNA was sampled during exponential growth (OD660 = 4) strains: LPA-0 (L-lysine hyperproducing strain C. glutamicum LYS-12 (Becker et al. 2011) + pClik5a_MCS), C. glutamicum LPA-5 (C. glutamicum LPA-0 + pClik5a_peftu_lat_proC)

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL32785

6 Samples

Download data: TXT, XLSX

(Submitter supplied) mRNA was sampled during exponential growth (OD660 = 4) strains: LPA-0 (L-lysine hyperproducing strain C. glutamicum LYS-12 (Becker et al. 2011) + pClik5a_MCS), C. glutamicum LPA-1A (C. glutamicum LPA-0 + pClik5a_peftu_lysDH_proC)

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL32785

6 Samples

Download data: TXT, XLSX

(Submitter supplied) Strains: non-producing refernece strain pXMJ19 (CR099 pXMJ19; Goldbeck et al., 2021) and Pediocin-producer pxMJ19 ped (CR099 pXMJ19 Ptac pedACDCg, Goldbeck et al., 2021) Pediocin-producing and non-producing strains of Corynebacterium glutamicum were compared in a whole genome microarray analysis setup in order to identify potential strain optimization targets

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL32940

6 Samples

Download data: TXT

(Submitter supplied) Efficient assimilation of renewable feedstocks is the cornerstone for achieving sustainable and economical microbial production of commodity chemicals. Unfortunately, most renewables are foreign to the cellular metabolism of classical industrial workhorses, resulting in unsatisfactory biomanufacturing performance. Here, Corynebacterium glutamicum was systematically engineered for rapid non-natural xylose metabolism and the underlying adaptations were elucidated by combining metabolic engineering, adaptive laboratory evolution and systems biology techniques. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30649

9 Samples

Download data: XLS

(Submitter supplied) RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. more...

Organism:

Streptococcus gordonii; Streptococcus sobrinus; Lacticaseibacillus casei; Streptococcus mutans UA159; Streptococcus sanguinis; Streptococcus mutans; Streptococcus oralis; Corynebacterium matruchotii

Type:

Expression profiling by high throughput sequencing

11 related Platforms

33 Samples

Download data: CSV, XLSX

(Submitter supplied) In an evolutionary experiment on high hemin concentrations, a frameshift mutation in the ChrS gene was figured out to be striking in survival at high hemin concentrations (Ala245fs). Apart from high upregulation of heme exporter hrtB, microarrays should reveal further different controlled genes compared to the WT.

Organism:

Pseudomonas putida KT2440; Corynebacterium glutamicum; Bacillus subtilis subsp. subtilis str. 168; Gluconobacter oxydans 621H; Escherichia coli str. K-12 substr. MG1655

Type:

Expression profiling by array

Platform:

GPL32387

3 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL29897

1214 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of C. glutamicum wild-type and mutant strains grown under different conditions.

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL29897

287 Samples

Download data

(Submitter supplied) Transcriptional profiling of C. glutamicum wild-type and mutant strains grown under different conditions.

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL29897

927 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Investigation of the impact of an overexpression of cg1978 on gene expression under standard conditions (CGXII-Glucose)

Organism:

Escherichia coli; Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032; Pseudomonas putida KT2440; Bacillus subtilis subsp. subtilis str. 168; Gluconobacter oxydans

Type:

Expression profiling by array

Platform:

GPL26911

3 Samples

Download data: GPR

(Submitter supplied) We assessed changes in gene expression in response to manganese availability for the human pathogen Corynebacterium diphtheriae. Total RNA was harvested from wild-type C. diphtheriae strain 1737 and an isogenic ΔmntR (dip0619) grown in semi-defined metal-limited media (mPGT) without or with 5 µM manganese chloride supplementation. Three biological replicates were prepared and five genes were assessed by real-time PCR to validate the array results: dip0124, dip0169, dip0615, dip1923, and dip2261. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL30087

12 Samples

Download data: TXT

(Submitter supplied) RNA degradation is a crucial process in bacterial cells for maintaining proper transcriptome homeostasis and coping with changing environments. A specialized ribonuclease known as RNase J (RnJ) participates in mRNA turnover in many Gram-positive bacteria; however, nothing is known about this process in Corynebacterium diphtheriae, nor is the identity of this RNase. We report here that C. diphtheriae DIP1463 encodes a predicted RnJ homolog, comprised of an N-terminal beta-lactamase domain, followed by beta-CASP and C-terminal domains. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29654

6 Samples

Download data: XLSX

(Submitter supplied) Differential gene expression analysis of C. glutamicum C1 in presence of 3 mM indole-alanine dipeptide compared to control conditions without indole-alanine dipeptide. C. glutamicum C1 cells were cultivated in CGXII minimal medium with 40 g per litre glucose in presence or absence of 3 mM indole-alanine dipeptide and harvested during exponential phase (o.d.600 6).

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29269

6 Samples

Download data: TXT

(Submitter supplied) Differential gene expression analysis of C. glutamicum ATCC 13032 in presence of 2.5 mM indole compared to control conditions without indole. C. glutamicum ATCC 13032 cells were cultivated in CGXII minimal medium with 40 g per litre glucose in presence of 2.5 mM indole and harvested during exponential phase (o.d.600 4).

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29269

2 Samples

Download data: TXT

(Submitter supplied) DNA microarray analysis was performed to check whether the addition and/or oxidation of L-arabinose to the growth medium of G.oxydans 621H possibly affects short-term global gene expression.

Organism:

Gluconobacter oxydans; Pseudomonas putida KT2440; Escherichia coli; Corynebacterium glutamicum; Bacillus subtilis subsp. subtilis str. 168; Gluconobacter oxydans 621H

Type:

Expression profiling by array

Platform:

GPL28615

1 Sample

Download data: GPR

(Submitter supplied) Previous findings have demonstrated that the NADH/NAD+ ratio has a strong impact on the glycolytic flux in C. glutamicum under anaerobic conditions in the absence of external electron acceptors. During an attempt to rewire anaerobic metabolism to achieve high yield formation of ethanol, we inactivated the malate dehydrogenase and lactate dehydrogenase in a C. glutamicum strain expressing pyruvate decarboxylase and alcohol dehydrogenase, to eliminate formation of the by-products succinate and lactate, respectively. more...

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28023

6 Samples

Download data: TXT

(Submitter supplied) In this study, we analyzed the regulation of ƴ-aminobutyrate (GABA) utilization in Corynebacterium glutamicum by the PucR-type transcriptional regulator GabR and by alternative nitrogen and carbon sources.

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29058

1 Sample

Download data: XLS

(Submitter supplied) Cell proliferation is achieved by numerous enzyme reactions. Temperature governs the activity of each enzyme, which overall determines the optimal growth temperature. Synthesizing useful chemicals and fuels utilizes only part of the metabolic pathways, especially the central metabolic pathways such as glycolysis and TCA cycle to metabolize glucose. However, the optimal temperature for the activity of the central metabolic pathways is inconclusive whether it is correlated with the optimal temperature for cell proliferation. more...

Organism:

Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL25854

3 Samples

Download data: TXT