GEO DataSets

Analysis of CD4+ T cell differentiation by expression profiling of subpopulations of CD4+ cells representing 5 successive stages of differentiation: intrathymic T progenitors, double positive thymocytes, single positive thymocytes, naïve T cells from cord blood, and naïve T cells from adult blood.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 5 development stage, 3 tissue sets

Platform:

GPL97

Series:

GSE1460

15 Samples

Download data: CEL

(Submitter supplied) Subpopulations of human fetal thymocyte and circulating naïve T cells were obtained through FACS sorting, including CD3-CD4+CD8- intrathymic T progenitor cells (ITTP), CD3intCD4+CD8+ "double positive" thymocytes (DP), CD3highCD4+CD8- "single positive" thymocytes (SP4), CD3+CD4+CD8-CD45RA+CD62L+ naïve T cells from cord blood (CB4+), and CD3+CD4+CD8-CD45RA+CD62L+ naïve T cells from adult blood (AB4+). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS785 GDS786

Platforms:

GPL96 GPL97

30 Samples

Download data: CEL

Analysis of CD4+ T cell differentiation by expression profiling of subpopulations of CD4+ cells representing 5 successive stages of differentiation: intrathymic T progenitors, double positive thymocytes, single positive thymocytes, naïve T cells from cord blood, and naïve T cells from adult blood.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 5 development stage, 3 tissue sets

Platform:

GPL96

Series:

GSE1460

15 Samples

Download data: CEL

(Submitter supplied) Gene expression profiling has opened new directions of investigation in scientific research, however, the need for substantial amount of biological material to perform these experiments limits significantly its application range. Here, we have developed procedures to perform microarray analysis on amplified cDNA from single cells, including primary T lymphocytes, and applied this technology to the study of CD4 and CD8 lineage differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL4725 GPL4726 GPL4724

66 Samples

Download data: TXT

(Submitter supplied) We have performed high-throughput RNA sequencing to characterize the transcriptomes of primary single positive (SP) CD4+ and CD8+ T cells from infant thymic tissue, as well as primary CD4+ and CD8+ T cells from infant and adult peripheral blood, to enable the comparisons across tissues and ages

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL11154

18 Samples

Download data: CSV

(Submitter supplied) The decline in adaptive immunity, naïve T-cell output and a contraction in the peripheral T cell receptor (TCR) repertoire with age are largely attributable to thymic involution and the loss of critical cytokines and hormones within the thymic microenvironment. To assess the molecular changes associated with this loss of thymic function, we used cDNA microarray analyses to examine the transcriptomes of thymocytes from mice of various ages ranging from very young (1 month) to very old (24 months). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

18 Samples

Download data: TXT

(Submitter supplied) Induced Pluripotent Stem Cell (iPSC)-derived T cells may provide future therapies for cancer patients, but those generated by current methods, such as the OP9/DLL1 system, have shown abnormalities that pose major barriers for clinical translation. Our data indicate that these iPSC-derived CD8 single positive T cells are more similar to CD4+CD8+ double positive T cells than to mature naïve T cells, display markers of developmental arrest and exhibit characteristics of innate-like immune cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: TXT

(Submitter supplied) Induced pluripotent stem cells (iPSC) might provide an unlimited source of T cells for a variety of clinical applications, including restoration of T cell immunity following stem cell transplantation and generation of minimally differentiated antigen-specific T cells for adoptive immunotherapy. However, current methods of differentiating T cells from iPSC using OP9/DLL1, an extrinsic source of Notch signaling, result in the accumulation of unconventional T cells either lacking CD8 co-receptors or expressing CD8αα homodimers. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

6 Samples

Download data: CEL