GEO DataSets

Analysis of Kyoto University Renal Cancer (KURC) xenograft tumor models, KURC1, KURC2, KURC3, treated with sunitinib, a small molecule inhibitor of receptor tyrosine kinases that is used to treat RC. Results provide insight into molecular mechanisms underlying acquired sunitinib resistance in RC.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 disease state, 3 individual sets

Platform:

GPL6244

Series:

GSE66346

9 Samples

Download data: CEL

(Submitter supplied) We established 3 types of primary xenograft models (KURC;Kyoto University Renal Cancer-1,2,3) derived from human renal cell carcinoma tissues, and 40 mg/day of sunitinib was orally administered. We performed microarray analysis to compare the gene expression profile of sunitinib-treated primary xenograft tumors (sensitive or resistant status) with that of vehicle-treated.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5815

Platform:

GPL6244

9 Samples

Download data: CEL

(Submitter supplied) Purpose: To identify tissue microRNAs predictive of sunitinib activity in patients with metastatic renal-cell carcinoma (MRCC) and to validate them in a cellular model. Selected microRNAs were studied in serum from MRCC patients and healthy individuals. Methods: We screened 673 microRNAs using TaqMan Low-density Arrays (TLDAs) in tumors from MRCC patients with extreme phenotypes of marked efficacy and resistance to sunitinib, selected from an identification cohort (n=41). more...

Organism:

Homo sapiens

Type:

Other

Platforms:

GPL13329 GPL13987

12 Samples

Download data: TXT

(Submitter supplied) We kept 2 types of primary xenograft models (KURC;Kyoto University Renal Cancer-1,3) derived from human renal cell carcinoma tissues, and 10 mg/kg of temsirolimus or vehicle was intraperitoneally administered.We performed microarray analysis to compare the gene expression profile of temsirolimus-treated primary xenograft tumors with that of vehicle-treated.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

9 Samples

Download data: CEL

(Submitter supplied) We kept a primary xenograft models (KURC;Kyoto University Renal Cancer-3) derived from human renal cell carcinoma tissues, and 10 mg/kg of temsirolimus or vehicle was intraperitoneally administered.We performed DNA methylation analysis to compare the methylation profile of temsirolimus-treated primary xenograft tumors with that of vehicle-treated.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

2 Samples

Download data: IDAT, TXT

(Submitter supplied) The role of epigenetic changes, relative to mutations, with targeted therapy

Organism:

Homo sapiens

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL11154

70 Samples

Download data: TXT

(Submitter supplied) The clinical response to sunitinib in advanced renal-cell carcinoma (RCC) is frequently limited in magnitude and duration due to drug resistance, but the underlying mechanisms remain elusive. To discover mechanisms of resistance, we developed drug-resistant cell lines and have their gene expressions profiled and compared. Results provide insight into the molecular mechanisms underlying sunitinib-resistance.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL19748

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9101

160 Samples

Download data: CEL, TXT

(Submitter supplied) Systematic somatic mutation screening of 4000 genes in human clear cell renal cell carcinoma. Information on corresponding somatic mutations in each sample can be found at http://www.sanger.ac.uk/genetics/CGP/Studies/.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9101

115 Samples

Download data: CEL, TXT

(Submitter supplied) Systematic somatic mutation screening of 4000 genes in human clear cell renal cell carcinoma. Information on corresponding somatic mutations in each sample can be found at http://www.sanger.ac.uk/genetics/CGP/Studies/. These samples were run at a different facility than VARI - scmmlab.com

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9101

45 Samples

Download data: CEL, TXT

(Submitter supplied) VHL mutations are the most common tumorigenic lesions in clear cell renal cell carcinoma (ccRCC) and result in continued activation of the HIF/VEGF pathway and uncontrolled cancer progression. Receptor tyrosine kinase (RTK) inhibitors such as sunitinib have been demonstrated to target tumorigenic signaling pathways, delay tumor progression and improve patient prognosis in metastatic renal cell carcinoma (mRCC). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: TXT

(Submitter supplied) A multiple receptor tyrosine kinase inhibitor, sunitinib, is a first-line therapy for clear cell renal cell carcinoma (CCRCC). Unfortunately, it has the major challenges of low initial response rate and resistance after about one year of treatment. Here we evaluated a microRNA (miRNA) and its target responsible for sunitinib resistance. Using miRNA profiling, we identified miR-96-5p upregulation in tumors from sunitinib-resistant CCRCC patients

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18402

11 Samples

Download data: TXT, XLSX

(Submitter supplied) Clear cell renal cell carcinoma is the most common type among kidney cancers, accounting for 80% of all kidney cancer cases and over 30% of patients have metastases at the time disease is diagnosed. The process od epithelial-mesenchymal transition is crucial for the development and progression of cancer, and here we wanted to check the expression of genes on different stages of tumor progression, and find which of them changes significantly with increased grade.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

60 Samples

Download data: CEL, CHP

(Submitter supplied) Purpose: Discovery of curative therapies for renal cell carcinoma (RCC) is hampered by lack of authentic preclinical models. Tumorgrafts, generated by direct implantation of patient-derived tissues into mice, have demonstrated superior ability to predict therapeutic response. We evaluated “tissue slice grafts” (TSGs) as an improved tumorgraft model of RCC. Experimental Design: Cores of fresh RCC were precision-cut at 300-μm and implanted under the renal capsule of RAG2-/-γC-/- mice. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

6 Samples

Download data: TXT

(Submitter supplied) Presently, there is a deficiency of effective therapies designed to target clear cell renal cell carcinoma (ccRCC), with poor prognosis resulting in patients with advanced disease. Additionally, there is a lack of molecular factors which can be remedially targeted resulting in tumor specific inhibition, and therefore current therapeutic approaches often produce adverse side effects in patients. We identified that Stearoyl-CoA desaturase 1 (SCD1) was consistently overexpressed in patient ccRCC samples, and further investigation of SCD1 as a potential molecular target for ccRCC intervention utilizing a SCD1 inhibitor (A939572) resulted in tumor specific growth inhibition and induction of cell death. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5094

Platform:

GPL570

8 Samples

Download data: CEL

Analysis of clear cell renal cell carcinoma (ccRCC) cells treated with A939572, a stearoyl-CoA desaturase 1 (SCD1) inhibitor. SCD1 is overexpressed in ccRCC.Results provide insight into the role of SCD1 in the pathogenesis of ccRCC.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 cell line sets

Platform:

GPL570

Series:

GSE41485

8 Samples

Download data: CEL

(Submitter supplied) Sunitinib resistance creates a major clinical challenge for the treatment of advanced clear cell renal cell carcinoma (ccRCC) and functional and metabolic changes linked to sunitinib resistance are not fully understood. We sought to characterize the molecular and metabolic changes induced by the development of sunitinib resistance in ccRCC by developing and characterizing the transcriptome of two human ccRCC cell lines resistant to sunitinib.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11154 GPL24676

12 Samples

Download data: TXT

(Submitter supplied) In order to establish a profile of gene expression of tumours treated with sunitinib, 4T1 breast tumours were allowed to establish in Balb/C mice for 1 week. The mice were then treated with 40 mg/kg Sunitinib in PBS and 3.72% DMSO (treatemt group) or PBS-DMSO vehicle alone (no treatment group), daily for the period of the experiment, after which the tumours were resected homogenised and subjected to CD31 microbead separation of endothelial cells and bulk tissue, both of which were subjected to agilent microarray analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

32 Samples

Download data: TXT

(Submitter supplied) While early stages of clear cell renal cell carcinoma (ccRCC) are curable, survival outcome for metastatic ccRCC remains poor. The purpose of the current study was to apply a new individualized bioinformatics analysis (IBA) strategy to these transcriptome data in conjunction with Gene Set Enrichment Analysis of the Connectivity Map (C-MAP) database to identify and reposition FDA-approved drugs for anti-cancer therapy. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3921 GPL9197

8 Samples

Download data: CEL, CHP

(Submitter supplied) The aim of this study was to investigate the effect of VEGF targeted therapy (sunitinib) on intratumoral heterogeneity (ITH) in metastatic clear cell renal cancer (mRCC).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

138 Samples

Download data: TXT