GEO DataSets

Analysis of cancer stem cells (CSCs) isolated from non-small cell lung cancer cell line H460 and allowed to redifferentiate under adherent conditions in serum-containing media for up to 24hr. Results provide insight into molecular mechanisms underlying the differentiation of H460-derived CSCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 growth protocol, 4 time sets

Platform:

GPL4133

Series:

GSE54712

8 Samples

Download data: GPR

(Submitter supplied) Transcription profile of cancer stem cells isolated from human non-small cells lung cancer (NSCLC) H460 cells. The profile of H460 cells that were made resistant to cisplatin after a single treatment with the drug was also determined. Both profiles were finally compared.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

8 Samples

Download data: TXT

(Submitter supplied) Cancer Stem Cells derived from human non-small cells lung cancer (NSCLC) H460 cells were differentiated by culturing them in the presence of Foetal Bovine Serum under adherent conditions. Changes in gene expression profiles were determined at 3, 9 and 24 hours of differentiation

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5410

Platform:

GPL4133

8 Samples

Download data: GPR

(Submitter supplied) This study was designed to understand the transcriptomic composition and the biological functions of cancer stem cells isolated from non-small cell lung cancer line (NSCLC) Putative lung cancer stem cells were isolated from cancer cell lines based on expression of known stem cell surface markers: CD166, CD44 and EpCAM using the Fluorescence Activated Cell Sorter (FACS).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

15 Samples

Download data: CEL, CHP

(Submitter supplied) Cancer metastasis remains an important unsolved problem. Matrix metalloproteinases (MMPs) have been shown to promote cancer cell transformation, migration, invasion, and metastasis through alteration of the extracellular microenvironment, and alter intracellular signaling and genome status. In addition, recent studies have shown intracellular and intranuclear localization, as well as roles of MMPs. In the present study, we examined gene expression signatures of high- and low-metastatic mouse colon cancer cells, and found that Mmp3 was expressed at the highest level in the high-metastatic cells. Profound nuclear localization of Mmps was found in primary explant sites as well as in areas of metastasis in lungs. In addition to the native 50-kDa Mmp3, a short 25-kDa PEX domain and active Mmp3 dimer were found in metastatic cancer cells, indicating novel roles for these forms. Knockdown of Mmp3 attenuated cancer cell viability, migration, and invasion in vitro, along with metastasis in an in vivo transplantation model, as well as cancer cell migration and invasion. These findings suggest that MMPs including intracellular, short, and dimerized forms are involved with malignant progression of cancer, thus they may be suitable as biomarkers and therapeutic targets.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21810

3 Samples

Download data: TXT

(Submitter supplied) BACKGROUND: Cancer stem-like cells were isolated from human H460 non-small cell lung cancer cells by limiting dilution assays and by their holoclone morphology, followed by assessment of their self-renewal capacity, tumor growth, vascularity, and tumor blood perfusion. H460 holoclones were used to implant H460 holoclone-derived tumors, which grew slower than parental H460 tumors, but displayed significant increases in microvessel density and tumor blood perfusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

4 Samples

Download data: TXT

(Submitter supplied) Cancer metastasis is a fetal problem that claims life of over 90% of cancer patients. It is hypothesized that cancer stem cells (CSCs) mediate cancer metastasis and such cells are often resistant to chemotherapy. Studying BRCA1 associated cancers, we found that CSCs form fillopodia and protrusions enriching for active forms of ezrin/radixin/moesin proteins and they have a much higher potential to metastasize than non-CSCs. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP