GEO DataSets

Temporal analysis of microdissected islets of NOD.Scid mice injected with diabetogenic CD4+BDC2.5 (BDC) T cells, then injected with anti-interferon-γ monoclonal antibody which protected the mice from developing diabetes. Results provide insight into the role of INF-γ role in autoimmune diabetes.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 2 protocol, 5 time sets

Platform:

GPL1261

Series:

GSE12389

8 Samples

Download data: CEL

(Submitter supplied) We demonstrate diverse roles of interferon–gamma (IFN-γ) in the induction and regulation of immune-mediated inflammation using a transfer model of autoimmune diabetes. The diabetogenic CD4+BDC2.5 (BDC) T cell clone upon transfer into NOD.scid mice induced destruction of islets of Langerhans leading to diabetes. Administration of a neutralizing antibody to IFN-γ (H22) resulted in long term protection (LTP) from diabetes, with inflammation but persistence of a significant, albeit decreased numbers of β-cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4034

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) Analysis of gene expression levels in ex-vivo and p79-stimulated splenic CD4+ T cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL14828

12 Samples

Download data: TXT

(Submitter supplied) In an accompanying paper we found specific localization of diabetogenic T cells only to islets of Langerhans bearing the specific antigen. Instrumental in the specific localization was the presence of intra-islet dendritic cells bearing the β-cell-peptide-MHC complex. Here we report that the entry of diabetogenic CD4 T cells very rapidly triggered inflammatory gene expression changes in islets and vessels by up-regulating chemokines and adhesion molecules. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4115 GDS4116

Platform:

GPL6246

20 Samples

Download data: CEL, CHP

Analysis of pancreatic islets after injection of diabetogenic BDC CD4 T cells into nondiabetic NOD Rag1-/- recipients, 24 to 48 hrs post-transfer. Results provide insight into molecular mechanisms underlying the T-cell entry induced state of receptivity of islets to subsequent immunological insults.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL6246

Series:

GSE26147

13 Samples

Download data: CEL, CHP

Analysis of islet cells after injection of diabetogenic 3A9 CD4 T cells into nondiabetic B10.BR IP-HEL recipients, 8 to 24 hrs post-transfer. Results provide insight into molecular mechanisms underlying the T-cell entry induced state of receptivity of islets to subsequent immunological insults.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol, 3 time sets

Platform:

GPL6246

Series:

GSE26147

7 Samples

Download data: CEL, CHP

(Submitter supplied) BDC2.5/NOD mice were treated with cyclophosphamide to induce type 1 diabetes. Their pancreatic islets were analyzed before treatment (Day 0) and as treatment progressed (Days 1 through 3) Keywords = type I diabetes Keywords = cyclophosphamide Keywords = BDC2.5 Keywords = NOD Keywords = pancreas Keywords = islets Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1056

Platform:

GPL81

18 Samples

Download data

Analysis of pancreatic islets from BDC2.5/NOD transgenics after treatment with 200 mg/kg cyclophosphamide (CY) at various time points up to 3 days. CY induces diabetes in BDC2.5/NOD transgenics. Results provide insight into molecular events accompanying the progression from insulitis to diabetes.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL81

Series:

GSE2254

18 Samples

Download data

(Submitter supplied) CD4 T cells play a critical role in promoting the development of autoimmunity in Type 1 Diabetes (T1D). The diabetogenic CD4 T cell clone BDC-2.5, originally isolated from a non-obese diabetic (NOD) mouse, has been widely used to study the contribution of autoreactive CD4 T cells and relevant antigens to autoimmune diabetes. Recent work from our lab has shown that the antigen for BDC-2.5 T cells is a hybrid insulin peptide (2.5HIP) consisting of an insulin C-peptide fragment fused to a peptide from chromogranin A (ChgA), and that endogenous 2.5HIP-reactive T cells are major contributors to autoimmune pathology in NOD mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: TXT

(Submitter supplied) Type I IFN-signaling suppresses an excessive IFN-{gamma} response and prevents lung damage and chronic inflammation following Pneumocystis (PC)-infection and clearance in CD4 T cell-competent mice. Type I IFN -signaling in pulmonary CD11c+ DCs and alveolar macrophages may prevent chronic inflammation following PC lung infection and clearance by suppressing an excessive IFN-g-response via the induction of SOCS1.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

(Submitter supplied) Tissue-specific autoimmune diseases are driven by activation of diverse immune cells in the target organs. However, the molecular signatures of immune cell populations over time in an autoimmune process remain poorly defined. Using single-cell RNA sequencing, we performed an unbiased examination of diverse islet-infiltrating cells during autoimmune diabetes in the nonobese diabetic mouse. The data revealed a landscape of transcriptional heterogeneity across the lymphoid and myeloid compartments. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17021 GPL24247

33 Samples

Download data: MTX, TSV

(Submitter supplied) Tissue-specific autoimmune diseases are driven by activation of diverse immune cells in the target organs. However, the molecular signatures of the immune cell populations over time in an autoimmune process remain poorly defined. Using single-cell RNA sequencing, we performed unbiased examination of diverse islet-infiltrating cells during autoimmune diabetes in the non-obese diabetic mouse. The data revealed a landscape of transcriptional heterogeneity across the lymphoid and myeloid compartments. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

7 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) Whole population RNASeq of pancreatic islet macrophages during autoimmune diabetes progression.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

26 Samples

Download data: TXT

(Submitter supplied) With the goal of identifying changes in gene expression in CD4(+) T cells during the development of diabetes in the nonobese diabetic (NOD) mouse, we used DNA microarrays to analyze gene expression in CD4(+) T cells from the pancreatic draining lymph nodes of NOD/BDC 2.5 T cell receptor transgenic and WT NOD mice at different ages. At 4 and 6 weeks of age, we found up-regulation of a number of genes that are known to be induced by IFN-alpha. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8291

16 Samples

Download data

(Submitter supplied) Interleukin (IL)-21 is essential for type 1 diabetes (T1D) development in the NOD mouse model. IL-21-expressing CD4 T cells are present in pancreatic islets where they contribute to disease progression. However, little is known about their phenotype and differentiation states. To fill this gap, we generated a novel IL-21 reporter NOD strain to further characterize IL-21+ CD4 T cells in T1D. IL-21+ CD4 T cells accumulate in pancreatic islets and recognize β-cell antigens. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: MTX, TSV

(Submitter supplied) The aim of this study was to explore what molecular and cellular processes predicate the conversion from insulitis to diabetes. The transcriptional profiles of CD45+ immune cells collected from pancreas of a cohort of age-matched female mice, which were scanned by MRI to determine the risk of diabetes development.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4347

Platform:

GPL6246

5 Samples

Download data: CEL

Analysis of pancreatic CD45+ cells from 10 week-old NOD females MRI-scanned to noninvasively visualize effects of pancreatic-islet inflammation. All NOD juveniles develop insulitis but vary highly in their progression to diabetes. Results provide insight into molecular basis of disease progression.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 5 individual sets

Platform:

GPL6246

Series:

GSE35096

5 Samples

Download data: CEL

(Submitter supplied) Defining the immunological landscape of human tissue is an important area of research, but challenges include the impact of tissue disaggregation on cell phenotypes and the low abundance of immune cells in many tissues. Here, we describe methods to troubleshoot and standardize Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq) for studies involving enzymatic digestion of human tissue. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24676

2 Samples

Download data: CSV, TAR