GEO DataSets

Analysis of vastus lateralis muscle biopsies from insulin-sensitive subjects, insulin-resistant subjects, and diabetic patients, following insulin treatment. Results provide insight into the molecular basis of insulin action in skeletal muscle and the underlying defects causing insulin resistance.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 disease state sets

Platform:

GPL91

Series:

GSE22309

110 Samples

Download data: CEL

(Submitter supplied) Insulin is a potent pleiotropic hormone that affects processes such as cellular growth, differentiation, apoptosis, ion flux, energy expenditure, and carbohydrate, lipid, and protein metabolism. We used microarrays to detail the global programme of gene expression underlying the influence of insulin in human skeletal muscle collected from different human individuals including 20 insulin sensitive, 20 insulin resistant and 15 diabetic patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3715

Platform:

GPL91

110 Samples

Download data: CEL, TXT

(Submitter supplied) Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3271

6 Samples

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(Submitter supplied) This study was undertaken to test the hypothesis that short term exposure (4 hours) to physiologic hyperinsulinemia in normal, healthy subjects without a family history of diabetes would induce a low grade inflammatory response, independently of glycemic status. We performed euglycemic hyperinsulinemic (80 mU/m2/min) clamps in 12 healthy, insulin sensitive subjects with no family history of diabetes followed by biopsies of the vastus lateralis muscle taken basally and after 30 and 240 minutes of insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3181

Platform:

GPL96

36 Samples

Download data: CEL

Analysis of skeletal muscles from normal, healthy glucose-tolerant individuals exposed to acute physiological hyperinsulinemia for up to 4 hours. Results suggest a low-grade inflammatory response which may provide insight into the etiology of insulin resistance in type 2 diabetes and obesity.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 12 individual, 3 time sets

Platform:

GPL96

Series:

GSE9105

36 Samples

Download data: CEL

(Submitter supplied) Type 2 diabetes mellitus (DM) is characterized by insulin resistance and pancreatic beta-cell dysfunction. In high-risk subjects, the earliest detectable abnormality is insulin resistance in skeletal muscle. Impaired insulin-mediated signaling, gene expression, and glycogen synthesis, and accumulation of intramyocellular triglycerides have all been linked with insulin resistance, but no specific defect responsible for insulin resistance and DM has been identified in humans. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL80

20 Samples

Download data: CEL

(Submitter supplied) 6 lean humans were submitted to a 3 hours hyperinsulinemic-euglycemic clamp. Skeletal muscle biopsies were taken before and after the clamp. Set of arrays that are part of repeated experiments

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6991

6 Samples

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(Submitter supplied) We examined global mRNA expression using cDNA microarrays in skeletal muscle of humans before, and 3h and 48h after a single bout of exhaustive endurance exercise (cycling). Keywords: Time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1679

Platform:

GPL3457

8 Samples

Download data: GPR

(Submitter supplied) 8,432 randomly chosen cDNAs from a 40k Research Genetics human cDNA library were amplified under standard conditions (GeneAmp PCR System 9700) with the help of a volumetric robot (Tecan/Genesis, RSP150 and Tomtec/Quadra 384). Amplified products were transferred to a 384-well plates, purified transferred to 384-well print plates (Genetix), dried and resuspended in print buffer (3xSSC, 1.5M Betaine). more...

Organism:

Homo sapiens

2 DataSets

3 Series

15 Samples

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Expression profiling of vastus lateralis muscles 3 and 48 hours after a 75-minute bout of high-intensity cycling. Results identify transcriptional pathways activated in skeletal muscles recovering from endurance exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 time sets

Platform:

GPL3457

Series:

GSE4247

8 Samples

Download data: GPR

(Submitter supplied) Experiment designed to identify differences in gene expression patterns in previously sedentary individuals before an endurance exercise training program. This series compares 2 groups of individuals that display high and low insulin sensitivity (SI) response (HSIR and LSIR) after 20 weeks of exercise (a 4-fold difference in SI response). There were no differences before training for SI, sex, age, BMI or body fat in the individuals integrating the LSIR and HSIR groups. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2234

Platform:

GPL1412

6 Samples

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Comparison of vastus lateralis muscles of previously sedentary individuals with improved insulin sensitivity (SI) after 20 weeks of exercise to those with no change in SI. Results provide insight into the mechanisms involved in the improvement in SI induced by regular exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 protocol sets

Platform:

GPL1412

Series:

GSE1718

6 Samples

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(Submitter supplied) Culturing myotubes from skeletal muscle (SM) biopsies enables investigating transcriptional defects and assaying therapeutic strategies. This study compares the transcriptome of aneurally cultured human SM cells versus that of tissue biopsies. We determined the transcriptomic differences between tissue and cultured SM samples from five individuals. In cultured myotubes compared to the tissue, 1216 genes were regulated: 583 down and 633 up. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6104

10 Samples

Download data: TXT

(Submitter supplied) Diagnosis of fatal hypothermia is considered to be difficult in forensic practice. In this study, in order to identify novel molecular markers of fatal hypothermia, we made rat models of mild, moderate and severe hypothermia, and performed body temperature-dependent gene expression analysis in illiopsoas muscle using next-generation sequencing.

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19052

12 Samples

Download data: TXT