GEO DataSets

Analysis of Mre11 deficient cells at meiotic prophase during sporulation. Mre11 is important in homologous recombination, repair of DNA double strand breaks, activation of damage-induced checkpoint, and telomere maintenance. Results provide insight into the role of Mre11 in spore development.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 development stage, 4 genotype/variation sets

Platform:

GPL90

Series:

GSE6620

8 Samples

Download data: CEL

(Submitter supplied) Mre11, together with Rad50 and Xrs2/NBS, plays pivotal roles in homologous recombination, repair of DNA double strand breaks (DSBs), activation of damage-induced checkpoint, and telomere maintenance. Using DNA microarray assays to analyze yeast mutants (mre11delta, rad50delta, and spo11Y135F) defective for meiotic DSB formation, we demonstrate that the absence of Mre11 in yeast causes specific effects on regulation of a class of meiotic genes for spore development. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2663

Platform:

GPL90

8 Samples

Download data: CEL

(Submitter supplied) Meiotic recombination is of central importance for the proper segregation of homologous chromosomes and is initiated by DNA double-strand breaks (DSB) that depend on Spo11 and Mre11. Calibrated chromatin immunoprecipitation (ChIP) for Mre11 shows that Spo11 promotes Mre11 recruitment to chromatin, independent of DSB formation. A C-terminal deletion mutant deficient in Spo11 interaction severely reduces the association of Mre11 with meiotic chromatin. more...

Organism:

Nakaseomyces glabratus; Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL34086

12 Samples

Download data: TXT, XLSX

(Submitter supplied) Temperature is key for biological activities, but its role in meiotic recombination processes is less known. Here, we uncovered the patterns of meiotic recombination by monitoring the double strands DNA breaks in diploid strain ZK5 cells cultured at 14ºC, 30ºC, and 37ºC.

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by genome tiling array

Platform:

GPL23647

12 Samples

Download data: GPR

(Submitter supplied) Temperature is key for biological activities, but its role in meiotic recombination processes is less known. Here, we uncovered the patterns of meiotic recombination by monitoring the genotypes of diploid strain JSC22-1-derived spores formed at 14ºC, 30ºC, and 37ºC. These spores were analyzed by whole genome SNP microarray that can examine about 13,000 SNPs distinguishing W303-1A and YJM789 sequences throughout the genome.

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by genome tiling array; Genome variation profiling by SNP array

Platform:

GPL20144

84 Samples

Download data: GPR

(Submitter supplied) In eukaryotes, meiotic recombination events are distributed non-randomly in the genome with certain regions having high levels of recombination (hotspots) and others having low levels (coldspots). Species with similar DNA sequences (for example, chimpanzees and humans) can have strikingly different patterns of hotspots and coldspots. Below, using a microarray analysis that allows us to measure the frequency of the meiosis-specific double-strand DNA breaks (DSBs) of all 6000 yeast genes, we show that mutation of a single gene (SIR2), which encodes a histone deacetylase, significantly changes DSB frequencies of 12% of yeast genes, elevating DSBs of 5% and reducing DSBs of 7%. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL4414

24 Samples

Download data: GPR

(Submitter supplied) RNA transcript signals were profiled in WT (MMY718) and jhd2∆ (MMY1879) terminally sporulated cultures (20h of sporulation) using Affymetrix high resolution tiling microarrays.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by genome tiling array

Platform:

GPL13990

4 Samples

Download data: CEL, TXT

(Submitter supplied) WT (MMY718) and jhd2∆ (MMY1879) sporulating cell cultures were profiled for global nucleosome occupancy using Affymetrix high-resolution tiling arrays.

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by genome tiling array

Platform:

GPL13990

16 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

44 Samples

Download data: GPR

(Submitter supplied) Meiosis was compared in msh5-22 and wild type strains of C. cinerea at 6 time points spanning the meiotic timecourse. Abstract: The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in ten million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently so differentially expressed genes might not be directly involved in meiotic processes. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

24 Samples

Download data: GPR

(Submitter supplied) Meiosis was compared in rad50-1 and wild type strains of C. cinerea at 6 time points spanning the meiotic timecourse. Abstract: The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in ten million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently so differentially expressed genes might not be directly involved in meiotic processes. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

20 Samples

Download data: GPR

(Submitter supplied) Dormancy is colloquially considered as extending lifespan by being still. Starved yeasts form dormant spores that wake-up (germinate) when nutrients reappear but cannot germinate (die) after some time. What sets their lifespans and how they age are open questions because what processes occur - and by how much - within each dormant spore remains unclear. With single-cell-level measurements, we discovered how dormant yeast spores age and die: spores have a quantifiable gene-expressing ability during dormancy that decreases over days to months until it vanishes, causing death. more...

Organism:

Saccharomyces cerevisiae W303

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29253

24 Samples

Download data: TXT