GEO DataSets

Analysis of transgenic embryonic stem cells induced with doxycycline to express neurogenin 3 (Ngn3) and differentiated for 3 or 10 days as embryoid bodies. Ngn3 is required for pancreatic islet development. Results provide insight into Ngn3-regulated molecular events during development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell line, 3 development stage, 3 time sets

Platform:

GPL1261

Series:

GSE3653

16 Samples

Download data: CEL

(Submitter supplied) Expression of the proendocrine gene neurogenin 3 (Ngn3) is required for the development of pancreatic islets. In order to better characterize the molecular events regulated by Ngn3 during development, we have determined the expression profile of differentiating murine embryonic stem cells (mESCs) uniformly induced to overexpress Ngn3. An ESC line was created that allows for the induction of Ngn3 by adding doxycycline (Dox) to the culture medium. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2276

Platform:

GPL1261

16 Samples

Download data: CEL, XLS

(Submitter supplied) Expression from CD133+ cells isolated from adult human exocrine tissue was compared to a CD133-depleted cell population

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: DIFF

(Submitter supplied) Induced overexpression of Pdx1 in activin-induced endoderm population resulted in the upregulation of pancreas-related genes such as insulin 1 and 2 at day 20. To enhance the developmental progression from the pancreatic bud to the formation of the endocrine lineages, we next expressed neurogenin3 (Ngn3) together with Pdx-1. Induced overexpression of Pdx1 together with Ngn3 dramatically increased Insulin 1 mRNA by day 9 differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

10 Samples

Download data: TXT

(Submitter supplied) Aims/hypothesis: Duct cells isolated from adult human pancreas can be reprogrammed to express islet beta cell genes by adenoviral transduction of the developmental transcription factor neurogenin3 (Ngn3). In this study we aimed to fully characterize the extent of this reprogramming and intended to improve it. Methods: The extent of the Ngn3-mediated duct-to-endocrine cell reprogramming was measured employing genome wide mRNA profiling. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL97 GPL96

26 Samples

Download data: CEL

(Submitter supplied) Genes specific to Sox9+ pancreatic progenitors were identified by comparing the gene expression in embryonic and adult Sox9+ cells. We used microarray analysis to detail the global changes in gene expression as Sox9 positive embryonic pancreatic progenitors differentiatiate into adult ductal cells or the endocrine lineage.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4335

Platform:

GPL6246

15 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL4134 GPL7202

16 Samples

Download data: TXT

(Submitter supplied) Sox9 target genes were identified during the secondary transition stage of pancreas development by comparing gene expression in Sox9-ablated versus wild-type pancreata using microarray analysis.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

8 Samples

Download data: TXT

(Submitter supplied) Sox9 target genes were identified by comparing gene expression in Sox9-ablated versus wild-type pancreata using microarray analysis.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

8 Samples

Download data: TXT

Analysis of GFP positive cells from Sox9-EGFP pancreas isolated by FACS at various developmental stages: e10.5, e15.5, p23. Results provide insight into molecular mechanisms underlying differentiatiation of Sox9+ embryonic pancreatic progenitors into adult ductal cells or the endocrine lineage.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age, 3 cell type sets

Platform:

GPL6246

Series:

GSE34060

15 Samples

Download data: CEL

(Submitter supplied) This dataset consists of single-cell RNA-seq (10X) data from 4 embryonic stages (E12.5-15.5) of pancreatic epithelial cells from Neurogenin3 (Ngn3)-Venus fusion (NVF) homozygous mice. Endocrine progenitor cells (NVF+) were enriched by FACS cell sorting.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

4 Samples

Download data: H5, TAR

(Submitter supplied) An 11-point time course study on differentiating embryoid bodies from a murine J1 embryonic stem cell line. The time course includes 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 4 days, 7 days, 9 days and 14 days. Keywords: Time course

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2668 GDS2669

Platforms:

GPL339 GPL340

66 Samples

Download data: CEL, EXP

(Submitter supplied) An 11-point time course study comparing V6.5 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2671 GDS2672

Platforms:

GPL339 GPL340

66 Samples

Download data: CEL, EXP

(Submitter supplied) An 11-point time course study comparing R1 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2666 GDS2667

Platforms:

GPL340 GPL339

66 Samples

Download data: CEL, EXP

Analysis of V6.5 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE3231

33 Samples

Download data: CEL, EXP

Analysis of V6.5 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE3231

33 Samples

Download data: CEL, EXP

Analysis of J1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE3749

33 Samples

Download data: CEL, EXP

Analysis of J1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE3749

33 Samples

Download data: CEL, EXP

Analysis of R1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE2972

33 Samples

Download data: CEL, EXP

Analysis of R1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE2972

33 Samples

Download data: CEL, EXP