GEO DataSets

(Submitter supplied) RNA-Seq was used for the first time to investigate the effects of resistance exercise on transcriptome dynamics in slow myosin heavy chain (MHC) I and fast MHC IIa muscle fibers among 3 groups of men. Vastus lateralis muscle biopsies were obtained before and 4 hours after resistance exercise.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

81 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

99 Samples

Download data

(Submitter supplied) Skeletal muscle is a complex heterogeneous tissue comprised of diverse muscle fiber and non-fiber cell types that, in addition to movement, influences other systems such as immunity, metabolism and cognition. We investigated gene expression patterns of resident human skeletal muscle cells using both single-cell RNA-seq and RNA-seq of single muscle fiber dissections from vastus lateralis. We generated transcriptome profiles of the major multinucleated human skeletal muscle fiber-types as well as 11 human skeletal muscle mononuclear cell types, including immune, endothelial, pericyte and satellite cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

18 Samples

Download data: TXT

(Submitter supplied) Global microarray (HG U133 Plus 2.0) was used to investigate the effects of resistance exercise and resistance training on the skeletal muscle transcriptome profile of 28 young and old adults. Vastus lateralis muscle biopsies were obtained pre and 4hrs post resistance exercise in the beginning (untrained state) and at the end (trained state) of a 12 wk progressive resistance training program.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5218

Platform:

GPL570

110 Samples

Download data: CEL, CHP

(Submitter supplied) Global microarray (HG U133 Plus 2.0) was used for the first time to investigate the effects of resistance exercise on the transcriptome in slow-twitch myosin heavy chain (MHC) I and fast-twitch MHC IIa muscle fibers of young and old women. Vastus lateralis muscle biopsies were obtained pre and 4hrs post resistance exercise in the beginning (untrained state) and at the end (trained state) of a 12 wk progressive resistance training program.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5217

Platform:

GPL570

70 Samples

Download data: CEL, CHP

(Submitter supplied) Global microarray (HG U133 Plus 2.0) was used to investigate the basal level skeletal muscle transcriptome profile of young and old adults. One vastus lateralis muscle biopsy was obtained in the basal state from 36 different subjects.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5216

Platform:

GPL570

36 Samples

Download data: CEL, CHP

Analysis of vastus lateralis muscle biopsy samples from young (about 24 years old) and old (about 84 years old) adults before and after 12 weeks of progressive resistance training. Results provide insight into the molecular adaptation of the young and old skeletal muscle to resistance training.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 age, 2 gender, 4 time sets

Platform:

GPL570

Series:

GSE28422

110 Samples

Download data: CEL, CHP

Analysis of slow-twitch MHC I and fast-twitch MHC IIa muscle fibers from young and old women before and after 12 weeks of progressive resistance training. Fibers obtained from vastus lateralis. Results provide insight into the molecular adaptation of young and old MHC I and IIa fibers to exercise.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 age, 14 individual, 4 time, 2 tissue sets

Platform:

GPL570

Series:

GSE28392

70 Samples

Download data: CEL, CHP

Analysis of vastus lateralis muscle biopsy samples from young (about 25 years old) and old (about 78 years old) adults. Subjects had not taken part in formal exercise. Results provide insight into the effect of age on basal gene expression in the skeletal muscle.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 age, 2 gender sets

Platform:

GPL570

Series:

GSE25941

36 Samples

Download data: CEL, CHP

(Submitter supplied) Age-related sarcopenia is associated with a variety of changes in skeletal muscle. These changes are interrelated with each other and associated with systemic metabolism, the details of which, however, are largely unknown. Eicosapentaenoic acid (EPA) is a promising nutrient against sarcopenia and has multifaceted effects on systemic metabolism. Although several human studies have suggested that EPA supplementation protects against sarcopenia, the causal relationship of EPA supplementation and an increase of muscle strength has poor evidence in vivo. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28457

3 Samples

Download data: TXT

(Submitter supplied) Total RNA-seq profiling of skeletal muscle.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

24 Samples

Download data: CSV

(Submitter supplied) Purpose: Reduced Representation Bisulfite Sequencing (RRBS) DNA input requirements become a challenge when working with small pools of tissue-specific cell types. We describe an application of the RRBS method to assess DNA methylation on low-DNA input from human slow-twitch (MHC I) and fast-twitch (MHC IIa) skeletal muscle fibers. Methods : Fiber-type specific (MHC I and MHC IIa muscle fibers) total DNA was extracted from vastus lateralis muscle biopsies of 8 young physically active men (~25 yrs). more...

Organism:

Homo sapiens

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL16791

2 Samples

Download data: CSV

(Submitter supplied) While acute aerobic and resistance exercise stimulate a number of shared genes, each exercsie mode stimlutes a number of uniquely responsive genes, thus highlighting that different forms of exercise facilitate distinct molecular responses in skeletal muscle.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

29 Samples

Download data: TXT

(Submitter supplied) Fast and slow skeletal muscles show different characteristics and phenotypes. This data obtained from microarray includes the comparison of normal fast plantaris and slow soleus muscles of adult rats. Characters of slow muscle are strongly dependent on the level of muscular activity. Denervation silences the muscular activity. Therefore, we determined the effects of denervation on gene expression in slow soleus muscle of adult rats.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL14797

3 Samples

Download data: TXT

(Submitter supplied) Contains 90 Sinperca chuatsi miRNAs identified in fast and slow muscle fibers, and 60 miRNAs showed significantly different levels of expression.

Organism:

Danio rerio; Siniperca chuatsi

Type:

Non-coding RNA profiling by array

Platform:

GPL23235

6 Samples

Download data: XLS

(Submitter supplied) Male and female muscle primary cultures were obtained from healthy young individuals. Cultures were treated with either estrogen, testesterone or DMSO. Gene expression was measured as response to the treatment

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

30 Samples

Download data: TSV

(Submitter supplied) The molecular transducers of benefits from different exercise modalities remain incompletely defined. Here we report that 12 weeks of high-intensity aerobic interval (HIIT), resistance (RT), and combined exercise training enhanced insulin sensitivity and lean mass, but only HIIT and combined training improved aerobic capacity and skeletal muscle mitochondrial respiration. HIIT revealed a more robust increase in gene transcripts than other exercise modalities, particularly in older adults, although little overlap with corresponding individual protein abundance was noted. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

103 Samples

Download data: TSV

(Submitter supplied) Exercise training can stimulate the formation of fatty acid oxidizing slow-twitch skeletal muscle fibers which are inversely correlated with obesity, but the molecular mechanism underlying this transformation requires further elucidation. Here, we report that the downregulation of the mitochondrial disulfide relay carrier CHCHD4 in skeletal muscle by exercise training decreases the import of TP53-regulated inhibitor of apoptosis 1 (TRIAP1) into mitochondria, which reduces cardiolipin levels and promotes VDAC oligomerization. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: TXT