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| Status |
Public on Feb 01, 2020 |
| Title |
Isolated fiber-type transcriptional profiles in human skeletal muscle |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal muscle is a complex heterogeneous tissue comprised of diverse muscle fiber and non-fiber cell types that, in addition to movement, influences other systems such as immunity, metabolism and cognition. We investigated gene expression patterns of resident human skeletal muscle cells using both single-cell RNA-seq and RNA-seq of single muscle fiber dissections from vastus lateralis. We generated transcriptome profiles of the major multinucleated human skeletal muscle fiber-types as well as 11 human skeletal muscle mononuclear cell types, including immune, endothelial, pericyte and satellite cells. We delineated two fibro-adipogenic progenitor cell subtypes that may contribute to heterotopic ossification and muscular dystrophy fibrosis under pathological conditions. An important application of cell type signatures is for computational deconvolution of cell type specific gene expression changes using data from bulk transcriptome experiments. Analysis of transcriptome data from a 12 week resistance exercise training study using these human skeletal muscle cell-type signatures revealed significant changes in specific mononuclear cell-type proportions related to age, sex, acute exercise and training. This characterization of human skeletal muscle cell types will resolve cell-type specific changes in large-scale physical activity muscle transcriptome studies and can further the understanding of the diverse effects of exercise and the pathophysiology of muscle disease.
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| Overall design |
Muscle fibers were isolated from biopsies, MHC content of each fiber was determined by SDS-PAGE, and fibers of each fiber-type (I vs. IIa) were pooled for sequencing.
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| Contributor(s) |
Rubenstein AB, Smith GR, Raue U, Ruf-Zamojski F, Nair V, Zhou L, Zaslavsky E, Trappe S, Sealfon S |
| Citation(s) |
31937892, 38095016 |
| |
| Submission date |
May 09, 2019 |
| Last update date |
Jan 30, 2024 |
| Contact name |
Aliza Rubenstein |
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Street address |
1 Gustave L. Levy Pl
|
| City |
Manhattan |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE236600 |
Effects of lifelong exercise and age on transcriptome dynamics in MHC I and MHC IIa muscle fibers at baseline and in response to resistance exercise (all samples) |
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| Relations |
| BioProject |
PRJNA542159 |
| SRA |
SRP197371 |
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