GEO DataSets

(Submitter supplied) Mosquito saliva facilitates blood feeding through the anti-haemostatic, anti-inflammatory and immunomodulatory properties of its proteins. However, the potential contribution of non-coding RNAs to host manipulation is still poorly understood. We analysed small RNAs from Aedes aegypti saliva and salivary glands and show here that chikungunya virus-infection triggers both the siRNA and piRNA antiviral pathways with limited effects on miRNA expression profiles. more...

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19664

12 Samples

Download data: XLSX

(Submitter supplied) During blood feeding haematophagous arthropods inject into their hosts a cocktail of salivary proteins whose main role is to counteract host haemostasis, inflammation and immunity. However, animal body fluids are known to also carry miRNAs. To get insights into saliva and salivary gland miRNA repertoires of the African malaria vector Anopheles coluzzii we used small RNA-Seq and identified 214 miRNAs, including tissue-enriched, sex-biased and putative novel anopheline miRNAs. more...

Organism:

Anopheles coluzzii

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL18984 GPL25627

14 Samples

Download data: TXT

(Submitter supplied) We have generated transgenic Ae. aegypti that express a ZIKV-specific inverted repeat RNA intended to trigger the mosquito siRNA anti-viral immune pathway. To confirm that the transgene is expressed and processed, we performed small RNA sequencing on both Higgs White Eye (HWE) & anti-NS3/4A-ZIKV transgenic Ae. aegypti 24 hours post-bloodmeal

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22030

2 Samples

Download data: TXT

(Submitter supplied) The study provides a comparative of transcript levels in uninfected and CHIKV-infected Aedes aegypti derived Aag2 cells using RNA Seq

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22761

6 Samples

Download data: TXT

(Submitter supplied) Dengue viruses (DENV) are generally maintained in a cycle which requires horizontal transmission via their arthropod vector, Ae. Aegypti, to the vertebrate host. One important consequence of this process is the interference of these arboviruses with both invertebrate and vertebrate immune systems. While infection of vertebrates causes disease, the presence of DENV gives rise to life-long, persistent infection in mosquitoes. more...

Organism:

Aedes aegypti

Type:

Expression profiling by SAGE

Platform:

GPL10360

2 Samples

Download data

(Submitter supplied) The Flavivirus genus contains some of the most prevalent vector-borne viruses such as dengue, Zika and yellow fever viruses that cause devastating diseases in humans. However, the insect-specific clade of flaviviruses is restricted to mosquito hosts; albeit they have retained the general features of the genus such as genome structure and replication. The interaction between insect-specific flaviviruses (ISFs) and their mosquito hosts are largely unknown. more...

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14869

36 Samples

Download data: XLSX

(Submitter supplied) We performed combined transcriptional and chromatin state analyses and showed that these core piRNA clusters in Aedes mosquitoes do not have their own independent promoters, but depend on readthrough transcription from upstream genes for piRNA biogenesis. We further showed core piRNA clusters biogenesis depends on key factors, the mRNA exporter Nxf1, three Tudor proteins, Veneno, Tejas, Yb, and an evolutionarily conserved cochaperone Shutdown.

Organism:

Aedes aegypti

Type:

Genome binding/occupancy profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL26709 GPL22030

38 Samples

Download data: BED, BROADPEAK, NARROWPEAK