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| Status |
Public on Aug 26, 2020 |
| Title |
The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegypti |
| Organism |
Aedes aegypti |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We have generated transgenic Ae. aegypti that express a ZIKV-specific inverted repeat RNA intended to trigger the mosquito siRNA anti-viral immune pathway. To confirm that the transgene is expressed and processed, we performed small RNA sequencing on both Higgs White Eye (HWE) & anti-NS3/4A-ZIKV transgenic Ae. aegypti 24 hours post-bloodmeal
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| |
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| Overall design |
small RNA profiling of 15 midguts from HWE & transgenic anti-NS3/4A-ZIKV Ae. aegypti 24 hours post-bloodmeal
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| |
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| Contributor(s) |
Williams AE, Sanchez-Vargas I, Reid WR, Lin J, Franz AW, Olson KE |
| Citation(s) |
33142991 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI130085 |
Engineering resistance to Zika virus in Aedes aegypti for Cas9 driven population modification |
COLORADO STATE UNIVERSITY |
KENNETH E OLSON |
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| |
| Submission date |
Aug 25, 2020 |
| Last update date |
Nov 25, 2020 |
| Contact name |
Ken E Olson |
| E-mail(s) |
Kenneth.Olson@ColoState.EDU
|
| Organization name |
Colorado State University
|
| Department |
Microbiology, Immunology, Pathology
|
| Street address |
3185 Rampart Road
|
| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80521 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22030 |
Illumina NextSeq 500 (Aedes aegypti) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA659285 |
| SRA |
SRP278820 |
