GEO DataSets

(Submitter supplied) We have generated transgenic Ae. aegypti that express a ZIKV-specific inverted repeat RNA intended to trigger the mosquito siRNA anti-viral immune pathway. To confirm that the transgene is expressed and processed, we performed small RNA sequencing on both Higgs White Eye (HWE) & anti-NS3/4A-ZIKV transgenic Ae. aegypti 24 hours post-bloodmeal

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22030

2 Samples

Download data: TXT

(Submitter supplied) We report that ZIKV noncoding RNA (sfRNA) affect expression of mosquito genes upon ZIKV infection. We constructed sfRNA-deficient ZIKV mutant (xrRNA2') and conducted transcriptome-wide gene expression profiling of mosquitos infected with WT and xrRNA2' viruses. We found that sfRNA inhibits apoptosis in the infected tissues of Ae. aegypti by altering expression of mosquito genes that control cell death and survival.

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26709

9 Samples

Download data: TXT

(Submitter supplied) We sequenced the small RNA profiles in ZIKV-infected and non-infected Ae. aegypti mosquitoes at 2, 7 and 14 days post-infection. ZIKV induced an RNAi response in the mosquito with virus-derived short interfering RNAs dramatically increased in abundance post-infection. Further, we found 17 host miRNAs that were modulated by the ZIKV infection at all time points.

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23267

18 Samples

Download data: TXT

(Submitter supplied) Mosquito saliva facilitates blood feeding through the anti-haemostatic, anti-inflammatory and immunomodulatory properties of its proteins. However, the potential contribution of non-coding RNAs to host manipulation is still poorly understood. We analysed small RNAs from Aedes aegypti saliva and salivary glands and show here that chikungunya virus-infection triggers both the siRNA and piRNA antiviral pathways with limited effects on miRNA expression profiles. more...

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19664

12 Samples

Download data: XLSX

(Submitter supplied) Zika virus (ZIKV) of the Flaviviridae family is a recently emerged mosquito-borne virus that has been implicated in the surge of the number of microcephaly instances in south America. The virus is transmitted mainly by the mosquito Aedes aegypti that also vectors dengue virus. Considering rather recent rapid spread of the virus and its declaration as a global health emergency by the World Health Organization, little is known about the interactions of the virus with the mosquito vector. more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23267

18 Samples

Download data: TXT

(Submitter supplied) The impact of global climate change on the transmission dynamics of infectious diseases is the subject of extensive debate. The transmission of mosquito-borne viral diseases is particularly complex, with climatic variables directly affecting many parameters associated with the prevalence of disease vectors. While evidence shows that warmer temperatures often decrease the extrinsic incubation period of an arthropod-borne virus (arbovirus), exposure to cooler temperatures often predisposes disease vector mosquitoes to higher infection rates. more...

Organism:

Aedes aegypti; Aedes albopictus

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL14869 GPL17038

8 Samples

Download data: XLSX

(Submitter supplied) Viral interfering RNA (viRNA) has been identified from several viral genomes via directly deep RNA sequencing of the virus-infected cells, including zika virus (ZIKV). Once produced by endoribonuclease Dicer, viRNAs, similar to microRNAs, are loaded onto Argonaute (AGO) family proteins of the RNA-induced silencing complexes (RISCs) to pair with their RNA targets and then initiate cleavage of the target genes. more...

Organism:

Homo sapiens

Type:

Other; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL21290

4 Samples

Download data: XLSX

(Submitter supplied) We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito, Aedes aegypti. Under standard conditions, mRNA-derived endo-siRNAs were produced from the bidirectional transcription of tail-tail overlapping gene pairs. Upon infection with the alphavirus, Sindbis virus (SINV), another class of mRNA-derived endo-siRNAs was observed. Genes producing SINV-induced endo-siRNAs were not enriched for overlapping partners or nearby genes, but were enriched for transcripts with long 3'UTRs. more...

Organism:

Aedes aegypti

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL11087 GPL14594

3 Samples

Download data: TXT

(Submitter supplied) We performed combined transcriptional and chromatin state analyses and showed that these core piRNA clusters in Aedes mosquitoes do not have their own independent promoters, but depend on readthrough transcription from upstream genes for piRNA biogenesis. We further showed core piRNA clusters biogenesis depends on key factors, the mRNA exporter Nxf1, three Tudor proteins, Veneno, Tejas, Yb, and an evolutionarily conserved cochaperone Shutdown.

Organism:

Aedes aegypti

Type:

Genome binding/occupancy profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL26709 GPL22030

38 Samples

Download data: BED, BROADPEAK, NARROWPEAK