GEO DataSets

(Submitter supplied) Purpose: The goal of this study was to identify Staphylococcus aureus genes which are important during establishment of metastatic infections in bloodstream infections. Methods: Pooled mariner transposon mutant libraries were generated as previously described (PMID: 27185949). The libraries were sequenced on the Illumina HiSeq 2500 platform with the transposon-specific oligonucleotide primer Himar1-Seq. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19006

12 Samples

Download data: CSV

(Submitter supplied) The transcriptomes of Δrho mutant cells and of wild-type cells grown in RPMI medium with or without 40 or 80 µg/ml of BCM were analyzed using Agilent microarrays in order to analyze the effects of the Rho inhibitor bicyclomycin (BCM) treatment on antisense transcription and changes in the expression of protein coding genes. All antisense RNAs exhibiting higher levels in the rho mutant compared to the wild-type showed similar levels in BCM-treated wild-type cells (80 µg/ml BCM). more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325

Type:

Expression profiling by array

Platform:

GPL24872

12 Samples

Download data: TXT

(Submitter supplied) It remains unclear how the bacterial populations which colonise many healthy humans occasionally give rise to severe disease. Staphylococcus aureus represents one such population. Here we describe mutations in invasive S. aureus which arose during human colonisation and inactivated rsp, a transcription factor. RNA-Seq was used to generate mRNA profiles of genes controlled by rsp.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17452

12 Samples

Download data: TXT

(Submitter supplied) Transcriptional start sites determination of USA300 (JE2) by using dRNA-Seq

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19006

4 Samples

Download data: WIG

(Submitter supplied) Methicillin resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen chief amongst bloodstream infecting pathogens. MRSA produces an array of human specific virulence factors that may contribute to immune suppression. Here, we defined the response of primary human phagocytes to infection with MRSA using RNA-Seq. We found that the overall transcriptional response to MRSA was weak both in the number of genes and the magnitude of response. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL24676

114 Samples

Download data: TXT

(Submitter supplied) Background. Infections caused by Staphylococcus aureus are associated with significant morbidity and mortality and are an increasing threat not only in hospital settings. The expression of the staphylococcal virulence factor repertoire is known to be affected by the alternative sigma factor B (SigB). However, its impact during infection still is a matter of debate. Methods. Kidney tissue of controls or mice infected with S. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

24 Samples

Download data: CEL

(Submitter supplied) We report here that mutants for purR display altered transcriptional profiles compared to WT S. aureus at multiple stages of growth.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18481

8 Samples

Download data: TXT

(Submitter supplied) To study the roles of NWMN_0641, we used microarray to compare the transcriptome of the NWMN_0641 deletion strain with that of the wild-type Staphylococcus aureus Newman strain. Transcriptome of the NWMN_0641 deletion mutant strain and the wild-type Newman strain

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus str. Newman

Type:

Expression profiling by array

Platform:

GPL1339

6 Samples

Download data: CEL

(Submitter supplied) Investigation of mRNA expression level changes in a Staphylococcus aureus Mu50 delta-SAV1322 mutant, compared to the wild-type strain. A comparison of the wild-type and the mutant transcription profiles

Organism:

Staphylococcus aureus subsp. aureus Mu50

Type:

Expression profiling by array

Platform:

GPL22334

8 Samples

Download data: PAIR

(Submitter supplied) The Staphylococcus aureus two-component regulatory system, SrrAB, coordinates hypoxic responses during in vitro growth conditions. S. aureus Affymetrix GeneChips were used to compare S. aureus expression properties of wild type and isogenic ssrA mutant cells during aerobic growth conditions. S. aureus Affymetrix GeneChips were also used to compare the S. aureus expression properties of wild type and isogenic ssrA mutant cells during hypoxic growth conditions. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

8 Samples

Download data: CEL, CHP

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of gene regulon. The goal of this study is to investigate the genes regulated by Rsp in MRSA BD02-25 Methods: mRNA profiles of wild-type (WT) and Rsp knockout (△Rsp) MRSA at mid-logarithmic growth phase (4h) were generated by deep sequencing, respectively in duplicate samples, using the Hiseq2000 (Illumina, CA) sequencer. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17452

4 Samples

Download data: TXT

(Submitter supplied) Staphylococcus aureus is an important human pathogen causing skin infection and many serious diseases such as pneumonia, sepsis, and toxic shock syndrome. We identified two anti-cancer drugs, streptozotocin (STZ) and floxuridine (FU), as promising lead compounds for further optimization into effective anti-virulence drugs against S. aureus infections. To understand the molecular mechanism of the in vivo efficacy of STZ and FU, we carried out RNA-seq. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24034

9 Samples

Download data: TXT

(Submitter supplied) The host protein calprotectin inhibits the growth of a variety of bacterial pathogens through metal sequestration in a process known as 'nutritional immunity'. Staphylococcus aureus growth is inhibited by calprotectin in vitro and calprotectin is localized in vivo to staphylococcal abscesses during infection. However, the staphylococcal adaptations that provide defense against nutritional immunity and the role of metal-responsive regulators are not fully characterized. more...

Organism:

Staphylococcus aureus

Type:

Other

Platform:

GPL19006

10 Samples

Download data: XLSX

(Submitter supplied) The host protein calprotectin inhibits the growth of a variety of bacterial pathogens through metal sequestration in a process known as 'nutritional immunity'. Staphylococcus aureus growth is inhibited by calprotectin in vitro and calprotectin is localized in vivo to staphylococcal abscesses during infection. However, the staphylococcal adaptations that provide defense against nutritional immunity and the role of metal-responsive regulators are not fully characterized. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27158

36 Samples

Download data: XLSX

(Submitter supplied) Staphylococcus aureus thymidine-dependent small-colony variants (TD-SCVs) are frequently isolated from patients with chronic S. aureus infections after long-term treatment with trimethoprim-sulfamethoxazole (TMP-SMX). In TD-SCVs, mutations of thymidylate synthase (thyA, TS), essential for DNA synthesis, occur. However, it has never been shown, that TMP-SMX is responsible for the induction and selection of TD-SCVs. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

18 Samples

Download data: CEL

(Submitter supplied) Staphylococcus aureus is an opportunistic pathogen that colonizes the anterior nares of about 30-50% of the population. Colonization is most often asymptomatic, however, self-inoculation through inhalation, ingestion, or an open wound, can give rise to potentially fatal infections of the deeper tissues and blood. Like all bacteria, S. aureus is able to sense and respond to environmental cues and modify gene expression to adapt to specific environmental conditions. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25144

9 Samples

Download data: XLSX

(Submitter supplied) CodY is a conserved broad acting transcriptional factor that regulates the expression of genes related to amino acid metabolism and virulence in Staphylococcus aureus. CodY target genes have been studied by using in vitro DNA affinity purification and deep sequencing (IDAP-Seq). In this study we performed the first in vivo determination of CodY target genes using a novel CodY monoclonal antibody in established ChIP-exo protocols. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25144

4 Samples

Download data: CSV

(Submitter supplied) The goal of this experiment was to analyse transcriptomic changes upon acidic pH-stress in different S. aureus strains, including a high persister clincal isolate CI-1149. In addition we aimed to identify differentally regulated pathways that can be linked to an increased persister phenotype as seen in our clinical strain.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24034

18 Samples

Download data: TXT

(Submitter supplied) AFN-1252 is an inhibitor of fatty acid biosynthesis. Gene expression profiles were generated by microarray analysis of S. aureus cells following treatment with AFN-1252, an inhibitor of fatty acid synthesis.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL1339

6 Samples

Download data: CEL

(Submitter supplied) LysR-type transcriptional regulators (LTTRs) are emerging as key circuit components in regulating microbial stress responses and are implicated in modulating oxidative stress in the human opportunistic pathogen Pseudomonas aeruginosa. The oxidative stress response encapsulates several strategies to overcome the deleterious effects of reactive oxygen species. However, many of the regulatory components and associated molecular mechanisms underpinning this key adaptive response remain to be characterised. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

6 Samples

Download data: CEL, CHP