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| Status |
Public on Feb 13, 2018 |
| Title |
Changes in relative transcript amounts caused by treatment of streptozotocin and floxuridine in S.aureus USA300 |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Staphylococcus aureus is an important human pathogen causing skin infection and many serious diseases such as pneumonia, sepsis, and toxic shock syndrome. We identified two anti-cancer drugs, streptozotocin (STZ) and floxuridine (FU), as promising lead compounds for further optimization into effective anti-virulence drugs against S. aureus infections. To understand the molecular mechanism of the in vivo efficacy of STZ and FU, we carried out RNA-seq. S. aureus USA300 was treated with 1 ug/mL (= 3.8 uM for STZ, 4 uM for FU) for 3 h. STZ treatment altered transcription of 829 genes (382 up-regulated; 447 down-regulated) whereas FU affected the transcription of 1192 genes (580 up-regulated; 612 down-regulated). Importantly, both compounds inhibited the transcription of the other virulence-regulatory systems such as Agr, Arl, and SarA. Therefore, the results indicate that the high in vivo efficacy of STZ and FU might be due to their repression of multiple virulence regulatory systems.
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| Overall design |
RNA was prepared from cultures of strains USA300_P23 (control), streptozotocin treated and floxuridine treated cells in TSB media at 37°C. Three independent biological replicates of RNA were prepared for each strain or condition. cDNA libraries were sequenced and the sequencing results were used as the data base for differential expression analysis.
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| Contributor(s) |
Taeok B, Won-Sik Y, Podicheti R, Rusch DB |
| Citation(s) |
29410445 |
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| Submission date |
Sep 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ram Podicheti |
| Organization name |
Indiana University
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| Street address |
1001 E 3rd St, JH 044
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| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
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| Platforms (1) |
| GPL24034 |
Illumina NextSeq 500 (Staphylococcus aureus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA408150 |
| SRA |
SRP118369 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104069_RAW.tar |
120.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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