GEO DataSets

(Submitter supplied) Drug discovery for novel anti-infectives is essential to meet the global health threat of antibiotic resistant bacterial infections, including those caused by Staphylococcus aureus1,2. Because ~90% of S. aureus infections involve skin and soft tissues (SSTIs)3,4, we hypothesized that developing anti-virulence therapeutics5,6 for SSTIs could minimize pressure on resistance development while sparing conventional antibiotics for control of systemic infections. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL8069

12 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27158

18 Samples

Download data

(Submitter supplied) The agr quorum-sensing system clearly links Staphylococcus aureus metabolism to virulence, but little is known about how agr alters metabolism to affect cell survival during severe stress. Recently, we reported that agr activity increases survival of bacteria during treatment with lethal concentrations of H2O2, a crucial host defense against S. aureus.  Here we report that protection by agr extends to growth resumption during the exit from stationary phase. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27158

12 Samples

Download data: TSV

(Submitter supplied) The agr quorum-sensing system clearly links Staphylococcus aureus metabolism to virulence, but little is known about how agr alters metabolism to affect cell survival during severe stress. Recently, we reported that agr activity increases survival of bacteria during treatment with lethal concentrations of H2O2, a crucial host defense against S. aureus.  Here we report that protection by agr extends to growth resumption during the exit from stationary phase. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27158

6 Samples

Download data: TSV

(Submitter supplied) The Staphylococcus aureus accessory gene regulator (agr) is a prototype quorum-sensing system in Gram-positive bacteria and a paradigmatic example of gene regulation by a small regulatory RNA, RNAIII. Using genome-wide transcriptional profiling in the community-associated methicillin-resistant (CA-MRSA) strain MW2, we demonstrate here that in contrast to the current model of target gene regulation by agr, a large subset of agr-regulated genes is controlled independently of RNAIII. more...

Organism:

Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Staphylococcus epidermidis ATCC 12228; Chlamydia caviae GPIC

Type:

Expression profiling by array

Platform:

GPL4692

27 Samples

Download data: CEL, CHP

(Submitter supplied) Staphylococcus hominis is frequently isolated from human skin and we hypothesize that it may protect the cutaneous barrier from opportunistic pathogens. We determined that S. hominis makes six unique auto inducing peptide (AIP) signals that inhibit the major virulence factor accessory gene regulator (agr) quorum sensing system of Staphylococcus aureus. We solved and confirmed the structures of three novel AIP signals in conditioned media by mass spectrometry, then validated synthetic AIP activity against all S. more...

Organism:

Staphylococcus hominis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32101

6 Samples

Download data: TXT

(Submitter supplied) The fungal metabolite apicidin acts as a quorum sensing inhibitor in Staphylococcus aureus. Here we use RNA-sequencing to examine the effect of apicidin on gene expression, comparing to untreated wild-type and an agr quorum sensing mutant.

Organism:

Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17544

9 Samples

Download data: CSV

(Submitter supplied) Exploration of the the transcriptomic consequences of the complete deletion of agrA gene in Staphylococcus lugdunensis. Overall, the ΔagrA deletion significantly affected the expression of 400 genes. Among them, 149 were upregulated and 251 were downregulated.

Organism:

Staphylococcus lugdunensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29710

8 Samples

Download data: TAB

(Submitter supplied) Aim: the goal of this study is to compare Quorum sensing related gene expression in MRSA cultures incubated with an aza-derivative

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24034

4 Samples

Download data: XLSX

(Submitter supplied) Compound VU0026921 is antimicrobial to S. aureus in a manner modulated by Mn. To elucidate information regarding the mechanism of action of VU0026921, we performed RNA sequencing of S. aureus exposed to VU0026921 in the presence or absence of excess Mn

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26273

12 Samples

Download data: TXT

(Submitter supplied) Manganese (Mn) is an essential micronutrient critical for the pathogenesis of Staphylococcus aureus, a significant cause of human morbidity and mortality. Paradoxically, excess Mn is toxic, therefore maintaining intracellular Mn homeostasis is required for survival. To identify gene candidates that contribute to manganese detoxification, we compared the transcriptional response of S. aureus cells exposed to 1 mM MnCl2 and those that were untreated.

Organism:

Staphylococcus aureus subsp. aureus str. Newman

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25976

6 Samples

Download data: TXT

(Submitter supplied) Transcriptional cofactors YAP/TAZ have recently been found to support autophagy and inflammation, which are part of cell autonomous immunity and are critical in antibacterial defense. Here, we studied the role of YAP against Staphylococcus aureus using CRISPR/Cas9-mutated HEK293 cells and a primary cell-based organoid model. We found that S. aureus infection increases YAP transcriptional activity, which is required to reduce intracellular S. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL31975

12 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL21493 GPL24247 GPL17021

42 Samples

Download data: BED, CSV, MTX, TSV, XLSX

(Submitter supplied) In order to explore and compare the transcriptomic profile of hypodermal adventitia fibroblasts, fibroblasts were isolated via flow cytometry mouse hypodermis and were subjected to RNA-sequencing analysis.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

12 Samples

Download data: XLSX

(Submitter supplied) In order to explore and compare the transcriptomic profile of leucocytes and stromal cells present in the dermis and hypodermis of C57BL/6 mice, isolated cells from each of these skin layers were subjected to single-cell RNA-sequencing analysis.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

8 Samples

Download data: MTX, TSV

(Submitter supplied) To explore the effect of cell-autonomous Igf1 expression on hypodermal macrophages, these cells were isolated from Csf1r-cre x Igf1-flox mice and subjected to single-cell ATAC-sequencing.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: BED, CSV, TSV

(Submitter supplied) In order to explore and compare the transcriptomic profile of CCR2+ macrophages, CCR2- macrophages and dendritic cells present in the dermis and hypodermis of C57BL/6 mice, isolated cells were subjected to RNA-sequencing analysis.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: XLSX