GEO DataSets

(Submitter supplied) To analyze function of three Two-component systems SAOUHSC_00184-185, SAOUHSC_01313-1314, and SAOUHSC_01980-1981, we have employed whole genome microarray expression profiling to identify genes transcription changed in respective mutant strains. Target TCSs were replaced by ermB gene.

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325

Type:

Expression profiling by array

Platform:

GPL17776

4 Samples

Download data: TXT

(Submitter supplied) Complete reconstitution of the vancomycin-intermediate Staphylococcus aureus (VISA) phenotype of Mu50 was achieved by sequentially introducing mutations into five genes of a vancomycin-susceptible S. aureus (VSSA) strain ∆IP. Introduction of mutation Ser329Leu into vraS encoding the sensor histidine kinase of vraSR two-component regulatory (TCR) system and another mutation Glu146Lys into msrR, encoding putative methionine sulfoxide reductase regulator, raised vancomycin resistance to the level of heterogeneously vancomycin-intermediate S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL16176

19 Samples

Download data: PAIR

(Submitter supplied) WalKR is an essential two component regulatory system in S. aureus, thought to control cell wall metabolism. Using genome sequencing of 5 paired clinical isolates of vancomycin-susceptible and vancomycin-intermediate S. aureus we found frequent, but unique, mutations in this locus. To investigate the contribution of these mutations to vancomycin resistance allelic replacement WalK (G223D) and WalR (K208R) mutants were generated and compared to the parent strains. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10592

6 Samples

Download data: TXT

(Submitter supplied) The phenotype “intermediate vancomycin resistance” in Staphylococcus aureus (CLSI: MIC = 4-8 mg/L) has been assigned to changes that lead to alterations in cell wall synthesis and morphology. Most vancomycin intermediately resistant S. aureus (VISA) strains are characterised by an increased cell wall thickness as a consequence of an activated cell wall biosynthesis and decreased autolysis. The purpose of this study was to analyse the genetic basis of the vancomycin resistance mechanism of the clinical VISA isolate SA137/93A and its spontaneous mutant strain SA137/93G. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus N315

Type:

Expression profiling by array

Platforms:

GPL5421 GPL5431

8 Samples

Download data: GPR

(Submitter supplied) Telavancin is a novel semi-synthetic lipoglycopeptide derivative of vancomycin with a dual mode of action. This study sought to understand knowledge of mechanisms of decreased susceptibility in laboratory-derived mutant. There were multiple significant changes in the transcriptome of TlvDSMED1952 indicating an altered overall physiology of the strain, likely decreased virulence, and changes in cell wall and membrane properties, probably due to multiple genetic changes in the strain.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10591

3 Samples

Download data: TXT

(Submitter supplied) Staphylococcus aureus is a notorious bacterial pathogen that causes a broad range of human diseases, and isolates that are resistant to several antibiotic classes including last resort antibiotics like vancomycin and daptomycin complicate the situation. We characterized S. aureus VC40, a strain that shows full resistance to vancomycin (MIC of 64 µg/ml) and daptomycin (MIC of 4 µg/ml) as well as a decreased susceptibility to further cell wall active agents. more...

Organism:

Staphylococcus aureus subsp. aureus VC40; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10537

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Staphylococcus aureus subsp. aureus str. JKD6008

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL31256 GPL34143

18 Samples

Download data: TXT

(Submitter supplied) Small RNAs have been found to control a broad range of bacterial phenotypes including tolerance to antibiotics. Vancomycin tolerance in multidrug resistance Staphylococcus aureus is correlated with dysregulation of small RNAs although their contribution to antibiotic tolerance in poorly understood. RNA-RNA interactome profiling techniques are expanding our understanding of sRNA-mRNA interactions in bacteria; however, determining the function of these interactions for hundreds of sRNA-mRNA pairs is a major challenge. more...

Organism:

Staphylococcus aureus subsp. aureus str. JKD6008

Type:

Other

Platform:

GPL34143

10 Samples

Download data: TXT

(Submitter supplied) Small RNAs have been found to control a broad range of bacterial phenotypes including tolerance to antibiotics. Vancomycin tolerance in multidrug resistance Staphylococcus aureus is correlated with dysregulation of small RNAs although their contribution to antibiotic tolerance in poorly understood. RNA-RNA interactome profiling techniques are expanding our understanding of sRNA-mRNA interactions in bacteria; however, determining the function of these interactions for hundreds of sRNA-mRNA pairs is a major challenge. more...

Organism:

Staphylococcus aureus subsp. aureus str. JKD6008

Type:

Other

Platform:

GPL34143

2 Samples

Download data: TXT

(Submitter supplied) Small RNAs have been found to control a broad range of bacterial phenotypes including tolerance to antibiotics. Vancomycin tolerance in multidrug resistance Staphylococcus aureus is correlated with dysregulation of small RNAs although their contribution to antibiotic tolerance in poorly understood. RNA-RNA interactome profiling techniques are expanding our understanding of sRNA-mRNA interactions in bacteria; however, determining the function of these interactions for hundreds of sRNA-mRNA pairs is a major challenge. more...

Organism:

Staphylococcus aureus subsp. aureus str. JKD6008

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31256

6 Samples

Download data: TXT

(Submitter supplied) To determine the role of the second gene in the vra operon (vraT) in the stress response to oxacillin, we compared the oxacillin induction profiles in a vraT deletion strain to that of a vraSR mutant in a USA300 MRSA strain background.

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL1339

18 Samples

Download data: CEL

(Submitter supplied) Daptomycin is a lipopeptide antibiotic that has recently been approved for treatment of Gram-positive bacterial infections. The mode of action of daptomycin is not yet entirely clear. To further understand the mechanism transcriptomic analysis of changes in gene expression in daptomycin-treated Staphylococcus aureus was carried out. The expression profile indicated that cell wall stress stimulon member genes (B. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL6059

6 Samples

Download data: TXT

(Submitter supplied) Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) which has been shown to increase the susceptibility of various bacteria to antimicrobials and demonstrated to have broad antimicrobial activity. This study describes transcriptome alterations in S. aureus strain COL grown with diclofenac and characterizes the effects of this NSAID on antibiotic susceptibility in laboratory, clinical and diclofenac reduced-susceptibility (DcRS) mutant S. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5879

4 Samples

Download data: TXT

(Submitter supplied) WalKR is a two-component system that is essential for viability in Gram-positive bacteria that regulates the all-important autolysins. Here we show a T101M mutation in walR confers a defect in autolysis, a thickened cell wall and decreased sensitivity to antibiotics that target the lipid II cycle, a phenotype that is reminiscent of the clinical resistance form known as vancomycin intermediate-resistant Staphylococcus aureus. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30817

9 Samples

Download data: TXT