Similar studies for GEO DataSets (Select 200035844) - GEO DataSets

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Items: 20

Select item 2000358441.

Dicer1 deletion in myeloid-committed progenitors causes neutrophil dysplasia and blocks macrophage/dendritic cell development in mice

(Submitter supplied) MiRNAs have the potential to regulate cellular differentiation programs. However, miRNA-deficiency in primary hematopoietic stem cells (HSCs) results in HSC depletion in mice, leaving the question of whether miRNAs play a role in early-lineage decisions unanswered. To address this issue, we deleted Dicer1, which encodes an essential RNaseIII enzyme for miRNA biogenesis, in murine CCAAT/enhancer-binding protein alpha (C/EBPA)-positive myeloid-committed progenitors in vivo. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS4504
Platform:: GPL1261
9 Samples

Download data: CEL, CHP

Series

Accession:: GSE35844

ID:: 200035844

Select item 45042.

Dicer1 deletion effect on bone marrow granulocyte-macrophage progenitors

Analysis of bone marrow-derived GMPs from Dicer1-deficient, C57BL/6 females. Dicer1 encodes an RNaseIII enzyme critical for miRNA biogenesis. Dicer1-deficient GMPs are defective in myeloid development in vitro. Results provide insight into the role of miRNAs in myeloid-lineage specification.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 3 genotype/variation sets

Platform:: GPL1261
Series:: GSE35844
9 Samples

Download data: CEL, CHP

DataSet

Accession:: GDS4504

ID:: 4504

Select item 2001660653.

CD62L expression level determines the cell fate of myeloid progenitors in mice and humans

(Submitter supplied) Through gene expression analysis of CMPs and GMPs at single cell levels, we identified CD62L as a marker to reveal the heterogeneity of CMPs and GMPs. We confirmed that the CD62L-low CMPs represent ‘bona fide’ CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-neg GMPs as the most immature subsets in GMPs and Ly6c+CD62L-low and Ly6c+CD62L-high GMPs are skewed to neutrophil and monocyte differentiation, respectively. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21273
21 Samples

Download data: TXT

Series

Accession:: GSE166065

ID:: 200166065

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000294534.

Expression data from miR-223KO and miR-223WT bone marrow cells

(Submitter supplied) Total bone marrow (BM) from miR-223 knockout (mir-223-/-) and wildtype (miR-223+/+) mice 21 was extracted, prestimulated for 2 days. Then, the BM cells were simultaneously cotransduced with MSCV-Hoxa9-pgk-neomycin and a MSCV-Meis1-IRES-YFP by co-cultivation with irradiated (4,000 cGy) viral producers. HoxA9-Meis1 transduced cells were sorted for YFP expression and continuously selected with neomycin (1.4 mg/ml). more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
8 Samples

Download data: CEL, TXT

Series

Accession:: GSE29453

ID:: 200029453

Select item 2000409655.

DICER1 hotspot mutations cause defective miRNA processing

(Submitter supplied) Recurrent somatic hotspot mutations of DICER1 appear to be clustered around each of four critical metal binding residues in the RNase IIIB domain of DICER1. This domain is responsible for cleavage of the 3’ end of the 5p-miRNA strand of a pre-mRNA hairpin. To investigate the effects of these cancer-associated “hotspot” mutations we engineered mouse Dicer1-deficient ES cells to express wild-type and an allelic series of the mutant human DICER1 variants. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
28 Samples

Download data: CEL

Series

Accession:: GSE40965

ID:: 200040965

Select item 2001133756.

Conditional DICER1 knockout hESCs

(Submitter supplied) We report the generation and characterization of DICER1-deficient hESCs. We uncover an unexpected requirement for DICER1 as well as essential pro-survival roles of members of the mir-302- 367 and mir-371- 373 clusters in hESCs. Our work provides a robust platform for interrogating microRNA function in hESC and differentiation.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
6 Samples

Download data: TXT

Series

Accession:: GSE113375

ID:: 200113375

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000889827.

RNA-seq of mouse myeloid progenitors reveals two independent pathways for monocyte production via GMPs and MDPs

(Submitter supplied) Granulocyte-monocyte progenitors (GMPs) and monocyte-dendritic cell progenitors (MDPs) produce monocytes during homeostasis and in response to increased demand during infection. Both progenitor populations are thought to derive from common myeloid progenitors (CMPs), and a hierarchical relationship (CMP-GMP-MDP-monocyte) is presumed to underlie monocyte differentiation. Here, however, we demonstrate that mouse MDPs arose from CMPs independently of GMPs, and that GMPs and MDPs produced monocytes via similar, but distinct, monocyte-committed progenitors. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
16 Samples

Download data: TXT

Series

Accession:: GSE88982

ID:: 200088982

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000650928.

Gene expression profiles of DKO172 cells expressing DICER1 wildtype or hotspot mutants

(Submitter supplied) DICER1 plays a critical role in microRNA (miRNA) biogenesis. Recurrent somatic “hotspot” mutations at four mental binding sites within the RNase IIIb domain of DICER1, were identified in ovarian sex cord-stromal tumors and have since been described in other pediatric tumors. In this study, we identified and characterized DICER1 hotspot mutations in endometrial cancers derived from The Cancer Genome Atlas (TCGA) and our local tumor bank. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL10787
12 Samples

Download data: TXT

Series

Accession:: GSE65092

ID:: 200065092

Select item 2001556439.

Pten and Dicer1 loss in the mouse uterus causes poorly-differentiated endometrial adenocarcinoma

(Submitter supplied) Endometrial cancer remains the most common gynecological malignancy in the United States. While the loss of the tumor suppressor, PTEN, is well studied in endometrial cancer, recent studies suggest that DICER1, the endoribonuclease responsible for miRNA genesis, also plays a significant role in endometrial adenocarcinoma. To examine the effects of DICER1 deletion on mRNA and miRNA expression in endometrial cancer, poly-A RNA sequencing (RNA-seq) and small RNA sequencing were performed. more...

Organism:: Mus musculus; Homo sapiens

Type:: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:: GPL19057 GPL18573
36 Samples

Download data: TSV

Series

Accession:: GSE155643

ID:: 200155643

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20012246810.

RNA-Seq of PreCFU-E and CFU-E progenitors from wild type and Scf mutants

(Submitter supplied) It has been shown previously that endothelial cells and LepR+ stromal cells are the main sources of SCF in vivo in the mouse bone marrow. We tested whether SCF from endothelial cells and/or LepR+ stromal cells is important for the maintenance of hematopoietic progenitors and erythroid progenitors in mouse bone marrow by conditional deletion of Scf from these two cell types. We discovered that Scf deletion from LepR+ stromal cells, but not endothelial cells, reduced the numbers of hematopoietic progenitors and erythroid progenitors in mice. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
18 Samples

Download data: TXT

Series

Accession:: GSE122468

ID:: 200122468

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20007116011.

Gene expression profiles of ovarian Sertoli-Leydig cell tumors

(Submitter supplied) Analysis of the global gene expression in ovarian Sertoli-Leydig cell tumor samples with or without DICER1 RNase IIIb domain mutation

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL14951
8 Samples

Download data: TXT

Series

Accession:: GSE71160

ID:: 200071160

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20004781312.

Pre-leukemic Cebpa mutant myeloid progenitors

(Submitter supplied) In this study, we use pre-malignant cells from different Cebpa mutant acute myeloid leukemia (AML) models. We have used conditional KO models (CreLoxP) and isolated hematopoietic cells shortly after induction of recombination, in order to look at pre-leukemic cells, which have acquired the first hit, but not yet undergone full malignant transformation.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
15 Samples

Download data: CEL

Series

Accession:: GSE47813

ID:: 200047813

Select item 20000658613.

DGCR8 is required for microRNA maturation

(Submitter supplied) To determine whether DGCR8 is required for maturation of all miRNAs, we performed miRNA microarray analysis. Using RNA from wild-type ES cells as our reference sample, we observed a global loss of miRNAs in DGCR8 knockout cells, but normal levels of expression in DGCR8 heterozygous cells. The similarity in expression levels between wild-type and heterozygous cells suggests that DGCR8 is not limiting in the maintenance of steady-state levels of miRNAs in ES cells. more...

Organism:: Mus musculus

Type:: Non-coding RNA profiling by array

Platform:: GPL4690
9 Samples

Download data: GPR

Series

Accession:: GSE6586

ID:: 200006586

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003491714.

IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array

Platforms:: GPL1261 GPL15097
13 Samples

Download data: CEL

Series

Accession:: GSE34917

ID:: 200034917

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003491515.

IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors (Illumina).

(Submitter supplied) While most blood lineages are assumed to mature through a single cellular and developmental route downstream of hematopoietic stem cells (HSCs), dendritic cells (DCs) can be derived from both myeloid and lymphoid progenitors in vivo. To determine how distinct progenitors can generate similar downstream lineages, we examined the transcriptional changes that accompany loss of in vivo myeloid potential as common myeloid progenitors (CMPs) differentiate into common dendritic cell progenitors (CDPs), and as lymphoid-primed multipotent progenitors (LMPPs) differentiate into all lymphoid progenitors (ALPs). more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL15097
8 Samples

Download data: TXT

Series

Accession:: GSE34915

ID:: 200034915

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003489216.

IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors (Affymetrix).

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
5 Samples

Download data: CEL

Series

Accession:: GSE34892

ID:: 200034892

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003889617.

Deregulated sex chromosome gene expression with male germ cell-specific loss of Dicer1

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Non-coding RNA profiling by array

Platforms:: GPL15734 GPL15733
11 Samples

Download data: CEL

Series

Accession:: GSE38896

ID:: 200038896

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003889518.

Deregulated sex chromosome gene expression with male germ cell-specific loss of Dicer1 (miRNA array data)

(Submitter supplied) MicroRNAs (miRNAs) are a class of endogenous, non-coding RNAs that mediate post-transcriptional gene silencing by inhibiting mRNA translation and promoting mRNA decay. DICER1, an RNAse III endonuclease encoded by Dicer1, is required for processing short 21-22 nucleotide miRNAs from longer double-stranded RNA precursors. Here, we investigate the loss of Dicer1 in mouse postnatal male germ cells to determine how disruptions in the miRNA biogenesis pathway may contribute to infertility. more...

Organism:: Mus musculus

Type:: Non-coding RNA profiling by array

Platform:: GPL15734
5 Samples

Download data: TXT

Series

Accession:: GSE38895

ID:: 200038895

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003889119.

Deregulated sex chromosome gene expression with male germ cell-specific loss of Dicer1 (gene array data)

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL15733
6 Samples

Download data: CEL, TXT

Series

Accession:: GSE38891

ID:: 200038891

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006130520.

Expression profile of control, Drosha or Dicer deleted LSKs

(Submitter supplied) To determine genes regulated independently of microRNAs in early haematopoietic progenitors (LSKs) we compared the expression profiles of Drosha or Dicer deficient LSKs and control. Those genes differentially expressed between Drosha or Dicer deficient LSKs are likely regulated indepedently of microRNAs as either Drosha or Dicer deletion will lead to a complete and equivalent loss of microRNAs.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
9 Samples

Download data: TXT

Series

Accession:: GSE61305

ID:: 200061305

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