GEO DataSets

(Submitter supplied) A comparative ChIP-chip analysis of TFIIB and NC2 in human B cells reveals that basal core promoter architectures control the equilibrium between NC2 and preinitiation complexes. We conducted a comparative ChIP-chip and gene expression analysis of TFIIB in human B cells and analyze associated core promoter architectures. TFIIB occupancy relates well to gene expression, with the vast majority of promoters being GC-rich and lacking defined core promoter elements. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6603

3 Samples

Download data: TXT

(Submitter supplied) The conserved core domain of the TATA binding protein (TBP) interacts with multiple partners forming the complexes required for transcription by RNA Polymerases I, II and III. We use genetically modified mouse embryonic fibroblasts to show that many TBP core domain mutants complement loss of endogenous TBP, but this often results in a slow growth phenotype. Two TBP mutations, R188E and K243E, disrupt the TBP-BTAF1 interaction and B-TFIID complex formation. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9250

7 Samples

Download data: BED

(Submitter supplied) In this study we show that disruption of NC2 function by shutting off either of its two protein subunits in yeast provokes an immediate increase in cryptic transcription around and inside any kind of RNA pol II genes.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by genome tiling array

Platform:

GPL19774

9 Samples

Download data: CEL, TXT

(Submitter supplied) Purpose: We want to know whether MINC(Mot1-Ino80C-NC2) suppress pervasive transcription at both euchromatin and heterochromatin Using next generation sequencing we show that Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 (MINC) colocalize on chromatin and cooperate to suppress pervasive transcription in S. cerevisiae and murine embryonic stem cells (mESCs). Conclusion: Our ChIP-Seq and mRNA-Seq data show that MINC regulates pervasive transcription in yeast and mammals

Organism:

Mus musculus; Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

4 related Platforms

117 Samples

Download data: BEDGRAPH, TXT, XLSX