GEO DataSets

(Submitter supplied) Aiming at the development of a micropollutant biosensor in the frame of the Micro-Ecological Life Support System Alternative (MELiSSA), a pilot study was initiated to identify triclosan (TCS)-responsive biomarker genes in the MELiSSA carbon-mineralizing microorganism, Rhodospirillum rubrum S1H. TCS is a biocide, commonly found in human excrements and is considered an emerging pollutant in wastewater and the environment. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

2 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rhodospirillum rubrum ATCC 11170; Rhodospirillum rubrum

Type:

Expression profiling by array

Platform:

GPL7256

6 Samples

Download data: GPR

(Submitter supplied) R. rubrum S1H inoculated on solid minimal media was sent to the ISS in September 2006 (BASE-A experiment). After 10 days flight, R. rubrum cultures returned back to Earth. These cultures were then subjected to both transcriptomic and proteomic analysis and compared with the corresponding ground control. Whole-genome oligonucleotide microarray and high throughput proteomics, which offer the possibility to survey respectively the global transcriptional and translational response of an organism, were used to test the effect of space flight. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

3 Samples

Download data: GPR

(Submitter supplied) R. rubrum S1H inoculated on solid agar rich media was sent to the ISS in October 2003 (MESSAGE-part 2 experiment). After 10 days flight, R. rubrum cultures returned back to Earth. These cultures were then subjected to both transcriptomic and proteomic analysis and compared with the corresponding ground control. Whole-genome oligonucleotide microarray and high throughput proteomics, which offer the possibility to survey respectively the global transcriptional and translational response of an organism, were used to test the effect of space flight. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

3 Samples

Download data: GPR

(Submitter supplied) 1. Oligos are synthesized and arrayed in Greiner 96-deep well plates. Each well contains more than 10nmol of 60-mer oligo and are resuspended in water to 10µM. 2. Resuspended oligos in water to 10 µM are rearrayed into 384-well (10µl transfered from 96 to 384 well plates), Genetix polystyrene V-bottom plates (cat# X6004). 3. 10µl of 2x spotting buffer is added to each well of the 384 plate; these plates are considered as spotting master plates. more...

Organism:

Rhodospirillum rubrum ATCC 11170

8 Series

32 Samples

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(Submitter supplied) In the frame of the European MELiSSA project, which aims to develop a closed biological life support system for forthcoming long term space exploration missions, the study of the alpha-proteobacterium Rhodospirillum rubrum S1H cultivated in space related environmental conditions has started. In the present work, the bacterium was grown using two different microgravity simulators, namely the Rotating Wall Vessel (RWV) and the Random Positioning Machine (RPM) and its response was evaluated at both the transcriptomic and proteomic levels using respectively a dedicated whole-genome microarray and high-througput proteomics. more...

Organism:

Rhodospirillum rubrum; Rhodospirillum rubrum ATCC 11170

Type:

Expression profiling by array

Platform:

GPL7256

2 Samples

Download data: GPR

(Submitter supplied) In the present study, we employed Affymetrix Staphylococcus aureus GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Staphylococcus aureus to triclosan, which involved initial growth inhibition and metabolism. Keywords: Transcriptome study; antimicrobial response; time course

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Dataset:

GDS2983

Platform:

GPL1339

15 Samples

Download data: CEL

Analysis of Staphylococcus aureus cultures treated for 10 or 60 minutes with 0.05 uM triclosan. At this concentration, the antimicrobial agent caused growth inhibition for about 10 min. Results provide insight into the initial molecular response of Staphylococcus aureus to triclosan.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array, count, 2 agent, 3 time sets

Platform:

GPL1339

Series:

GSE8861

15 Samples

Download data: CEL

(Submitter supplied) Triclosan is a biocidal active agent commonly found in domestic cleaning products, hand sanitizers, cosmetics and personal care products. It is used to control microbial contamination and has a broad-spectrum of activity against many Gram-positive and Gram-negative bacteria. The development of triclosan tolerance with potential cross resistance to clinically relevant antibiotics in zoonotic pathogens is of concern given the widespread use of this active agent in clinical, food processing and domestic environments. more...

Organism:

Escherichia coli; Escherichia coli O157

Type:

Expression profiling by array

Platform:

GPL3154

12 Samples

Download data: CEL

(Submitter supplied) Through chemical contamination of natural environments, microbial communities are exposed to many different types of chemical stressors; however, research on whole genome responses to this contaminant stress is limited. This study examined the transcriptome response of a common soil bacterium, Pseudomonas aeruginosa, to the common environmental contaminant pentachlorophenol (PCP). Cells were grown in chemostats at a low growth rate to obtain substrate-limited, steady-state, balanced-growth conditions. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

12 Samples

Download data

(Submitter supplied) The growth and persistence of rhizobia and bradyrhizobia in soils are negatively impacted by drought conditions. In this study, we used genome-wide transcriptional analyses to obtain a comprehensive understanding of the response of Bradyrhizobium japonicum to drought. Desiccation of cells resulted in the differential expression of 15 to 20% of the 8,480 B. japonicum open reading frames, with considerable differentiation between early (after 4 h) and late (after 24 and 72 h) expressed genes. more...

Organism:

Bradyrhizobium diazoefficiens USDA 110; Bradyrhizobium japonicum

Type:

Expression profiling by array

Platform:

GPL5341

18 Samples

Download data: GPR

(Submitter supplied) Exposure to common environmental chemicals, including those found in personal care products has been linked to mammary cancer at high doses in animal models. Their effects at low doses at levels comparable to human exposure, especially during critical windows of development remain poorly understood. Using a Sprague-Dawley rat model, we investigated the effects of of three environmental chemicals – diethyl phthalate (DEP), methyl paraben (MPB) and triclosan (TCS) – on the transcriptome of normal developing mammary glands at low doses mimicking human exposure. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

81 Samples

Download data: CEL, XLS

(Submitter supplied) RNAseq was performed on sixth generation anaerobic and aerobic R. rubrum cultures

Organism:

Rhodospirillum rubrum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26615

2 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of NTG-treated E. coli imp fabI(G93V) cells compared to control cells (E. coli imp fabI(G93V) ) in the absence or presence of sub-lethal concentration of triclosan.

Organism:

Escherichia coli

Type:

Expression profiling by array

Platform:

GPL5482

2 Samples

Download data: TXT