GEO DataSets

(Submitter supplied) Hemozoin phagocytosis results in immunomodulation. This study was designed to explore gene expression responses to 15(S)-HETE in LPS-stimulated RAW 264.7 cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

6 Samples

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(Submitter supplied) Hemozoin phagocytosis results in immunomodulation. In the present study, gene expression profiles were analyzed to identify transcriptional changes associated with two of the individual components of Hz in a model macrophage cell line. LPS-stimulated RAW 264.7 cells were exposed to the synthetic form of Hz, β-hematin (BH, 0.1 mg/mL) and HNE (35 μM) for 6 or 24 h. Keywords: variable treatment

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6246 GPL2995

24 Samples

Download data: CEL

(Submitter supplied) Paeony root has long been used for its anti-inflammatory effects. In this study, the effects of albiflorin, paeoniflorin, and paeonol, compounds from paeony root, on gene expression profiles were examined in macrophages. The RAW264.7 macrophages were treated with LPS in the presence or absence of albiflorin, paeoniflorin, or paeonol. Global mRNA expression levels were detected by using an oligonucleotide microarray platform covering the mouse whole genome. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

33 Samples

Download data: TXT

(Submitter supplied) Background: Toll-like family of receptors recognizes pathogen-associated molecular patterns (PAMPs) from different organisms. TLR4 is the receptor for bacterial lipopolysaccharide (LPS), dsRNA viral mimic poly(I:C) binds to TLR3, and bacterial CpG DNA signals through TLR9. TLR4 signaling is mediated by adaptor molecules Myd88 and TRIF while TLR3 pathway involves only the TRIF adaptor and TLR9 signals solely through Myd88. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL891

46 Samples

Download data: TXT

(Submitter supplied) We compared the gene expression patterns of macrophages infected with S. oralis wild type and SpxB KO, a strain that does not produce H2O2.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21810

8 Samples

Download data: TXT

(Submitter supplied) Using RNA-seq, we have reported that signaling crosstalk among IFN-γ and LPS is integrated at the level of transcriptome and have associated with TFs and TcoFs changes. In addition, we also argue that the transcriptional differences between BMDMs and RAW264.7 macrophage cell line as well as IL-4 and IL-13 on M2 macrophages activation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

25 Samples

Download data: TAB

(Submitter supplied) Triplicate samples of RAW 264.7 murine macrophages either untreated, stimulated with 100 ng/ml LPS for 18 hours, or constituitively over-expressing CstF-64 were analyzed by microarray using Affymetrix murine gene chip 430A. Keywords = RAW 264.7 macrophages Keywords = LPS Keywords = CstF-64 Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1285

Platform:

GPL339

9 Samples

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Analysis of RAW 264.7 macrophages treated with 100 ng/ml lipopolysaccharide (LPS) for 18 hours, or overexpressing polyadenylation factor CstF-64. LPS treatment increases CstF-64 expression. Results provide insight into post-transcriptional mechanisms affected by LPS in macrophages.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL339

Series:

GSE2002

9 Samples

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(Submitter supplied) Comparison of gene expression in wildtype and MyD88-/- C57BL/6J mouse macrophages treated with 10 ng/mL LPS for 2 hours versus media treated control macrophages, and, wildtype and MyD88-/- C57BL/6J mouse macrophages treated with live E. coli bacteria (log phase; 1 bact per 1 macrophage) for 2 hours versus media treated control macrophages. Cells from 4 mice of each geneotype were used and each individual provided its own control. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1705 GDS1706

Platform:

GPL1226

31 Samples

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Analysis of MyD88 null mutant macrophages treated with LPS or live E. coli. MyD88 transduces cell signaling events downstream of Toll-like receptors, a key component of host defense. Results suggest most of the host response to endotoxin or live bacteria is actually regulated independently of MyD88.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 genotype/variation sets

Platform:

GPL1226

Series:

GSE1405

15 Samples

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Analysis of MyD88 null mutant macrophages treated with LPS or live E. coli. MyD88 transduces cell signaling events downstream of Toll-like receptors, a key component of host defense. Results suggest most of the host response to endotoxin or live bacteria is actually regulated independently of MyD88.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 genotype/variation sets

Platform:

GPL1226

Series:

GSE1405

16 Samples

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(Submitter supplied) Our aim was to identify genes that were differentially expressed in microglia stimulated with Lipopolysaccharide, Luteolin, or both. Affymetrix microarrays were used to analyze RNA samples

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3613

Platform:

GPL1261

12 Samples

Download data: CEL

Analysis of lipopolysaccharide-activated microglial BV-2 cells treated with luteolin, a plant derived flavonoid. Luteolin possesses the ability to scavenge oxygen and nitrogen species, and exhibits anti-inflammatory activities. Results provide insight into the immuno-modulatory effects of luteolin.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL1261

Series:

GSE18740

12 Samples

Download data: CEL

(Submitter supplied) Among the multiple mechanisms that control the intensity and duration of macrophage activation, the development of a state of refractoriness to a second stimulation in cells treated with LPS has long been recognized. Release of inhibitory cytokines and alterations in intracellular signaling pathways may be involved in the development of LPS tolerance. Although a number of molecules have been implicated, a detailed picture of the molecular changes in LPS tolerance is still missing. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) To provide a global perspective on the relationships between macrophage activation programs and to understand how certain pathogens circumvent them, we used transcriptional profiling by genome wide microarray analysis to compare the responses of mouse macrophages following exposure to the intracellular parasites Trypanosoma cruzi and Leishmania mexicana, the bacterial product lipopolysaccharide (LPS), and the cytokines IFNG, TNF, IFNB, IL-4, IL-10, and IL-17. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7275

111 Samples

Download data: GPR

(Submitter supplied) LPS plus ISO and LPS plus PGE2 had non-additive effects on gene expression in RAW 264.7 cells Keywords: time course

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL254

88 Samples

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(Submitter supplied) LPS plus IFG and LPS plus 2MA had non-additive effects on gene expression in RAW 264.7 cells Keywords: time course

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL254

90 Samples

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(Submitter supplied) All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for either 1, 2, 4, 8, 16 or 48 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for either 1, 2, 4, 8, 16 or 48 hours. This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL254

36 Samples

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(Submitter supplied) The proinflammatory cytokine, TNFalpha is critical in maintaining liver homeostasis since it is a major determiner of hepatocyte life and death. Considering this, gene transcription profiling was examined in control and TNFalpha treated HepG2 cells. Results indicated that TNFalpha could significantly alter the expression of a significant number of genes; most of them were functionally distributed among molecular functions like catalytic activity, binding, molecular transducer activity, transporter activity, translation and transcription regulator activities or enzyme regulator activity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP