GEO DataSets

(Submitter supplied) Bacillus anthracis is a gram-positive, aerobic, spore-forming, rod-shaped bacterium which has recently been used as an agent of bioterrorism. Because there is a significant delay between the initial contact of the spore with the host and clinical evidence of disease, there appears to be temporary containment of the pathogen by the innate immune system. Contact with the human alveolar macrophage (HAM) plays a key role in the innate immune response to B. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

7 Samples

Download data: CEL

(Submitter supplied) The lung is the entry site for Bacillus anthracis in inhalation anthrax, the most deadly form of the disease. Spores must escape through the alveolar epithelial cell (AEC) barrier and migrate to regional lymph nodes, germinate and enter the circulatory system to cause disease. Several mechanisms to explain alveolar escape have been postulated, and all these tacitly involve the AEC barrier. In this study, we incorporate our primary human type I AEC model, microarray gene profiling and gene enrichment analysis to study the response of AEC to B. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

12 Samples

Download data: CEL

(Submitter supplied) Cultured keratinocytes treated with TNFa in the presence or absence of NFkB inhibitor; time course 1, 4, 24 & 48 hrs. Keywords = Epidermis Keywords = skin Keywords = keratinocytes Keywords = inflammation Keywords = NFkB Keywords = TNFa Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1289

Platform:

GPL8300

16 Samples

Download data: CEL, EXP

Analysis of epidermal keratinocytes treated with TNFalpha in the presence of 10 uM parthenolide, an NF-kappa B inhibitor. Cells examined at various time points up to 48 hours following TNFalpha treatment. Results identify NF-kappa B-dependent subset of TNFalpha-regulated genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 time sets

Platform:

GPL8300

Series:

GSE2489

16 Samples

Download data: CEL, EXP

(Submitter supplied) Edema factor of anthrax binds to the protective antigen (PA83) which cleaves to PA63 and PA20. The role of PA20 was examined using highthroughput gene expression analysis of human peripheral blood mononuclear cells (PBMC) exposed to PA20. Keywords: Toxicity determination

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3033

14 Samples

Download data: GPR

(Submitter supplied) TNF time course series of HelA tet off cells cultured in presence or absence of Dox TNF is a pro-inflammatory cytokine that controls expression of inflammatory genetic networks. Although the Nuclear Factor-kB (NF-kB) pathway is crucial for mediating cellular TNF responses, the complete spectrum of NF-kB dependent genes is unknown. In this study, we used a tetracycline-regulated cell line expressing an NF-kB inhibitor to systematically identify NF-kB dependent genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1212

Platform:

GPL8300

32 Samples

Download data

Temporal analysis of the effect of tumor necrosis factor (TNF) on gene expression in HeLa cells with NF-kappaB inactivated. NF-kappaB inactivated with 2 ug/ml doxycycline. Cells examined at various time points following stimulation with 25 ng/ml TNF. Results identify potential targets of NF-kappaB.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL8300

Series:

GSE2624

32 Samples

Download data

(Submitter supplied) Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4419

Platform:

GPL570

14 Samples

Download data: CEL

Analysis of alveolar macrophages purified from bilateral bronchoalveolar lavage after saline instillation into a lung segment followed by LPS instillation into the contralateral lung. Results provide insight into innate immune pathways activated in alveolar macrophages exposed to LPS in vivo.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 7 individual, 2 stress sets

Platform:

GPL570

Series:

GSE40885

14 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9052 GPL5175

26 Samples

Download data: BED, CEL

(Submitter supplied) Macrophages play a critical role in innate immunity, and the expression of early response genes orchestrate much of the initial response of the immune system. Macrophages undergo extensive transcriptional reprogramming in response to inflammatory stimuli such as Lipopolysaccharide (LPS). To identify gene transcription regulation patterns involved in early innate immune responses, we used two genome-wide approaches - gene expression profiling and chromatin immunoprecipitation-sequencing (ChIP-seq) analysis. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9052

18 Samples

Download data: BED

(Submitter supplied) Macrophages play a critical role in innate immunity, and the expression of early response genes orchestrate much of the initial response of the immune system. Macrophages undergo extensive transcriptional reprogramming in response to inflammatory stimuli such as Lipopolysaccharide (LPS). To identify gene transcription regulation patterns involved in early innate immune responses, we used two genome-wide approaches - gene expression profiling and chromatin immunoprecipitation-sequencing (ChIP-seq) analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

8 Samples

Download data: CEL

(Submitter supplied) The domestic pig (Sus scrofa) provides a large animal model for human innate immune responses and inflammation that is also economically important in its own right. Results: We demonstrate that macrophages can be harvested from 3 different compartments of the pig (lungs, blood and bone-marrow), cryopreserved and subsequently recovered and differentiated in CSF-1. We have performed surface marker analysis and gene expression profiling on macrophages from these compartments, comparing 25 animals from 5 different breeds and their response to lipopolysaccharide. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16569

140 Samples

Download data: CEL

(Submitter supplied) The role of infection in erythropoietic dysfunction is poorly understood. In children with P. falciparum malaria, the by-product of hemoglobin digestion in infected red cells (hemozoin) is associated with the severity of anemia which is independent of circulating levels of the inflammatory cytokine tumor necrosis alpha (TNF-alpha). To gain insight into the common and specific effects of TNF-alpha and hemozoin on erythropoiesis, we studied the gene expression profile of purified primary erythroid cultures exposed to either TNF-alpha (10ng/ml) or to hemozoin (12.5microgram/ml heme units) for 24 hours. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

4 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4044

424 Samples

Download data: TXT

(Submitter supplied) To understand the transcripts induced by TNFa and how these may interact in inflammatory networks, the transcriptome of HUCECs stimulated with TNFa was examined in 3 different pools each cosisting of 10 different HUVEC isolates.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4044

24 Samples

Download data: TXT

(Submitter supplied) Gene regulatory networks inferred from RNA abundance data have generated significant interest. Several high-profile studies have suggested that gene networks may be useful for identifying molecular pathways related to development, human disease and drug action. However despite, this potential, gene network approaches are used relatively infrequently and their use generally requires input from specialist bioinformaticians. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4044

400 Samples

Download data: TXT

(Submitter supplied) Gene expression was profiled in peripheral blood samples collected over three time points from patients during acute anaphylaxis and from healthy controls. Patients presented to the Emergency Department (ED) at Royal Perth Hospital with acute, moderately severe anaphylaxis. Samples were collected at ED arrival (T0), 1 hour later (T1), and 3 hours post arrival (T2).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17244

36 Samples

Download data: CEL

(Submitter supplied) This present study is the first to investigate the global changes in host gene expression during the interaction of human bronchial epithelial cells and live Alternaria spores. Human bronchial epithelial cells (BEAS2-B) were exposed to spores or media alone for 24 hours. RNA was collected from three biological replicates/treatment and used to assess changes in gene expression patterns using Affymetrix Human Genome U133 Plus 2.0 Arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP

(Submitter supplied) At present, with the accelerating pace of life, increase in work pressure, lack of exercise, irregularity of diet and rest, and problems of environmental pollution, people with immunodeficiency are gradually increasing in the modern population. Some Chinese herbal medicines can improve the immunity of the human body, and have less and mild side or toxic effects, so that the study on this kind of traditional Chinese medicines and its mechanism has become an urgent problem to be solved. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

6 Samples

Download data: TXT