GEO DataSets

(Submitter supplied) The subcellular localization of proteins is critical to their biological roles. Moreover, whether a protein is membrane-bound, secreted, or intracellular affects the usefulness of, and the strategies for, using a protein as a diagnostic marker or a target for therapy. We employed a rapid and efficient experimental approach to classify thousands of human gene products as either membrane-associated/secreted (MS) or cytosolic/nuclear (CN). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

5 related Platforms

19 Samples

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(Submitter supplied) Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. mRNA species bound to membrane-associated polysomes were separated from other mRNAs by sedimentation equilibrium or sedimentation velocity. The distribution of individual transcripts in the 'membrane-bound' and 'cytosolic' fractions was quantitated for thousands of genes by hybridization to DNA microarrays. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2773

1 Sample

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(Submitter supplied) Large-scale identification of secreted and membrane-associated gene products using DNA microarrays. This study is described more fully in Diehn M et al.(2000) Nat Genet 25:58-62 Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL179

1 Sample

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(Submitter supplied) RNA from MCF-7 cells was fractionated by sucrose density gradient centrifugation to separate RNA associated with membrane-bound polysomes from RNA associated with free polysomes. These two populations were hybridized in triplicate to U133A microarrays. Keywords: repeat sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS992

Platform:

GPL96

6 Samples

Download data: CEL

Identification of genes whose mRNA are translated from the endoplasmic reticulum (ER) in the breast cancer cell line MCF-7. Secreted and membrane-bound proteins are preferentially translated in the ER. Results provide insight into the association of membrane-bound proteins and breast cancer.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL96

Series:

GSE1400

6 Samples

Download data: CEL

(Submitter supplied) Membrane-associated, integral membrane and secreted proteins are of key importance in many cellular processes. For most of the 28 952 predicted proteins in Arabidopsis, the actual subcellular localisation has not been demonstrated experimentally. So far, their potential membrane-association has been deduced from algorithms that predict transmembrane domains and signal peptides. However, the comprehensiveness and accuracy of these algorithms is still limited. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL3357

12 Samples

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(Submitter supplied) Array hybridizations profiling diverse human tissues; for each array the human tissue RNA is hybridized against a universal reference RNA. Experiments 1-3 are replicates of universal reference RNA vs. genomic DNA hybridizations, used to estimate transcript abundance. Arrays 4-8 are tissue RNA vs. genomic DNA hybridizations, carried out to evaluate the method for estimating transcript abundance (see manuscript for details). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1827

8 Samples

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(Submitter supplied) Array hybridizations profiling diverse human tissues; for each array the human tissue RNA is hybridized against a universal reference RNA. Series_type: Logical set Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS1085 GDS1088

5 related Platforms

123 Samples

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Expression profiling of 115 samples from 35 different types of normal tissues. Samples obtained by surgery or autopsy, and evaluated by pathologists. Results provide insight into the molecular organization of diverse cell types, and provide a baseline for comparison to diseased tissues.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 4 tissue sets

Platform:

GPL1826

Series:

GSE2193

11 Samples

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Expression profiling of 115 samples from 35 different types of normal tissues. Samples obtained by surgery or autopsy, and evaluated by pathologists. Results provide insight into the molecular organization of diverse cell types, and provide a baseline for comparison to diseased tissues.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 20 tissue sets

Platform:

GPL1823

Series:

GSE2193

35 Samples

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(Submitter supplied) SNP Expression profiling of human breast cancer: 29 tumor samples, 4 pure normal breat samples and 8 lymphocytes samples Keywords: Human Cancer

Organism:

Homo sapiens

Type:

Genome variation profiling by SNP array

Platform:

GPL2005

41 Samples

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(Submitter supplied) Since the early stages of tumorigenesis involve adhesion, escape from immune surveillance, vascularization and angiogenesis, we devised a strategy to study the expression profiles of all publicly known and putative secreted and cell surface genes. We designed a custom oligonucleotide microarray containing probes for 3531 secreted and cell surface genes to study 5 diverse human transformed cell lines and their derivative xenograft tumors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1814

98 Samples

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(Submitter supplied) Time course analysis series in Development of the transcriptome from Drosophila melanogaster using the Heidelberg FlyArray. All stages were hybridized against embryonic stage 0-4 h as reference control. Keywords: time-course

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL517

24 Samples

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(Submitter supplied) see Vodkin et al., BMC Genomics 5:73 (2004) for details of cDNAs represented on the arrays and Thibaud-Nissen et al., Plant Physiol 132:118-136 (2003); Zou et al. Mol Plant Microbe Interat 18:1161-1174 (2005); Zabala and Vodkin, Plant Cell 17: 2619-2632 (2005) for use of cDNA arrays in soybean. Protocol: PCR amplified cDNA inserts printed with Genemachines Omnigrid

Organism:

Glycine max

6 Series

82 Samples

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(Submitter supplied) see Vodkin et al., BMC Genomics 5:73 (2004) for details of cDNAs represented and Thibaud-Nissan, et al., Plant Physiol 132: 118-136 (2003); and Zabala and Vodkin, Plant Cell 17: 2619-2632 (2005) for use of cDNA arrays in soybean Protocol: amplified cDNAs printed with Genemachines Omnigrid

Organism:

Glycine max

12 Series

191 Samples

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(Submitter supplied) The understanding of metastatic spread is limited and molecular mechanisms causing particular characteristics of metastasis are largely unknown. This comprises the extremely varying dormancy periods of tumor cells in the secondary organ after metastatic spread, represented by the disease-free survival (DFS) of the patients, or differing numbers of metastases in different patients. Knowing the molecular fundamentals of these phenomena would support the individual prediction of patients´ outcome and facilitate the decision for an appropriate monitoring and therapy regime. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

68 Samples

Download data: CEL