GEO DataSets

(Submitter supplied) Anti-estrogens and aromatase inhibitors are important drugs in the treatment of estrogen-dependent breast cancer. In order to investigate the effects of these drugs on gene expression in breast cancer cells, we treated estrogen receptor-positive MCF-7 cells, stably transfected with the aromatase gene (known as MCF-7aro cells), with testosterone, 17β-estradiol, two aromatase inhibitors (letrozole and anastrozole), and an anti-estrogen (tamoxifen). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1873

Platform:

GPL96

18 Samples

Download data: CEL, EXP

Analysis of estrogen receptor positive, aromatase transfected MCF-7 cells after treatment with an antiestrogen (AE) or an aromatase inhibitor (AI). AEs and AIs are used to treat estrogen-dependent breast cancer. Cells also treated with androgen which is converted to estrogen by aromatase.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 6 agent, 4 other sets

Platform:

GPL96

Series:

GSE2225

18 Samples

Download data: CEL, EXP

(Submitter supplied) The beneficial effect of the selective estrogen receptor modulator (SERM) tamoxifen in the treatment and prevention of breast cancer is assumed to be through its ability to antagonize the stimulatory actions of estrogen, although tamoxifen can also have some estrogen-like agonist effects. Here we report that in addition to these mixed agonist/antagonist actions, tamoxifen can also selectively regulate a unique set of more than 60 genes in estrogen receptor alpha (ERa)-positive MCF-7 human breast cancer cells which are minimally regulated by estradiol or raloxifene. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2367

Platform:

GPL96

17 Samples

Download data

Analysis of estrogen receptor alpha (ERalpha)-positive MCF-7 breast cancer (BC) cells infected with adenovirus-ERbeta and treated with tamoxifen (Tam). Tam is a selective ER modulator used in BC prevention and treatment. Results provide insight into Tam activity in the presence of both ERs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 2 infection sets

Platform:

GPL96

Series:

GSE4025

17 Samples

Download data

(Submitter supplied) Compare the expression pattern of 17b-estradiol responsive genes in parent, OHT-resistant and ICI-resistant breast cancer cells. Keywords: 17b-estradiol responsive genes, OHT resistance, Fulvestrand resistance

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data

(Submitter supplied) Resistance to endocrine therapy agents has presented a clinical obstacle in the treatment of hormone-dependent breast cancer. Our laboratory has initiated a study of microRNA regulation of signaling pathways that may result in breast cancer progression on aromatase inhibitors (AI). Microarray analysis of microRNA expression identified 115 significantly regulated microRNAs, of which 49 microRNAs were believed to be hormone-responsive. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL9081

23 Samples

Download data: TXT

(Submitter supplied) Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer. A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL, CHP

(Submitter supplied) MCF-7aro cells were used to generate a cell culture model system that is resistant to 3 aromatase inhibitors (AIs), letrozole, anastrozole and exemestane. For comparison, the MCF-7aro cells were also used to generate the tamoxifen-resistant cells as well as long-term estrogen deprived, LTEDaro. Affymetrix microarray analysis was performed to determine changes in gene expression that are unique to AI-resistance. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

42 Samples

Download data: CEL

(Submitter supplied) Transcriptome of 17 tru-cut biopsies and 13 matched surgical samples from ER+ breast tumor patients, treated for 3 months with Anastrozole were anayzed to identify a robust expression signature predictive of response to Anastrozole neoadjuvant treatment in ER+ breast cancer patients and, at the same time, to delineate treatment effects.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6480 GPL6848

60 Samples

Download data: TXT

(Submitter supplied) To investigate response or resistance to endocrine therapy, mice with targeted over-expression of Esr1 or CYP19A1 to mammary epithelial cells were employed, representing two direct pathophysiological interventions in estrogen pathway signaling. Both Esr1 and CYP19A1 over-expressing mice responded to letrozole with reduced HAN prevalence and decreased mammary epithelial cell proliferation. CYP19A1 over-expressing mice were tamoxifen-sensitive but Esr1 over-expressing mice were tamoxifen-resistant. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

17 Samples

Download data: TXT

(Submitter supplied) In the present investigation, we have exploited the opportunity provided by neoadjuvant treatment of a group of postmenopausal women with large operable or locally advanced breast cancer (in which therapy is given with the primary tumour remaining within the breast) to take sequential biopsies of the same cancers before and after 10-14 days or 90 days treatment with letrozole. RNA extracted from the biopsies has been subjected to Affymetrix microarray analysis and the data from paired biopsies interrogated to discover genes whose expression is most influenced by oestrogen deprivation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

176 Samples

Download data: CEL

(Submitter supplied) This series represents murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h. For 6 h exposures, the explants were cultured overnight on fibronectin coated 35mm dishes (Biocoat, Becton Dickinson Labware, Bedford, MA) in DMEM with 10% horse serum in order to allow for sufficient outgrowth of neural crest cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS773

Platform:

GPL81

27 Samples

Download data: CEL, EXP

Expression profiling of cranial neural crest exposed to 1 uM retinoic acid (RA) at various lengths of time up to 48 hours. Neural crest obtained from 8.5 days postcoitum ICR embryos. Results provide insight into developmental pathways affected upon exposure to teratogenic concentrations of RA.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 4 time sets

Platform:

GPL81

Series:

GSE1588

27 Samples

Download data: CEL, EXP

(Submitter supplied) Recent studies have identified clinical subtypes of breast tumors that arise from different cell types and have different outcomes in response to chemotherapy. Tumors derived from basal epithelium have a poorer prognosis than tumors derived from luminal epithelium. To gain insight into differences underlying this disparity, we treated cell lines derived from basal epithelium (immortalized human mammary epithelial cells) and those derived from luminal epithelium (MCF-7 and ZR-75-1) with two chemotherapeutics commonly used in the treatment of breast cancer. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS845 GDS846 GDS847 GDS848

Platform:

GPL550

75 Samples

Download data

Analysis of the response of luminal epithelium derived breast cancer line ZR-75-1 to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

19 Samples

Download data

Analysis of the response of luminal epithelium derived breast cancer line MCF-7 to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

19 Samples

Download data

Analysis of the response of basal epithelium derived breast cancer line HME-CC to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

19 Samples

Download data

Analysis of the response of the basal epithelium derived breast cancer line ME16C to either doxorubicin or 5-fluorouracil. Cells examined 12, 24, and 36 hours following treatment. Results provide insight into the response of breast cancers derived from different cell types to chemotherapeutics.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 agent, 3 time sets

Platform:

GPL550

Series:

GSE763

18 Samples

Download data

(Submitter supplied) In the present investigation, we have exploited the opportunity provided by neoadjuvant treatment of a group of postmenopausal women with large operable or locally advanced breast cancer (in which therapy is given with the primary tumour remaining within the breast) to take sequential biopsies of the same cancers before and after 10-14 days treatment with letrozole. RNA extracted from the biopsies has been subjected to Affymetrix microarray analysis and the data from paired biopsies interrogated to discover genes whose expression is most influenced by oestrogen deprivation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3116

Platform:

GPL96

116 Samples

Download data: CEL

Analysis of breast cancer tumors following treatment with letrozole for 14 days. The aromatase inhibitor letrozole is an anti-estrogen drug used to treat postmenopausal women with breast cancer. Results provide insight into the molecular mechanism of action of letrozole in breast cancer.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 58 individual sets

Platform:

GPL96

Series:

GSE5462

116 Samples

Download data: CEL