GEO DataSets

(Submitter supplied) Since macrophage activation can now be studied at a global level using modern microarray and proteomic analyses, discovery of novel macrophage activation genes is inevitable and important for understanding HIV-associated dementia (HAD). We isolated two different types of primary human macrophages, microglia and monocyte-derived macrophages (MDM) from brain tissue and whole blood respectively. The microarray analysis of differentially regulated macrophage activation genes reported here supports our previous assertions that the mixed glia (MIX) cultured in starvation conditions (DMEM alone) are a non-activated, or “quiescent”, tissue culture model for studying macrophage activation in the brain. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

14 Samples

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(Submitter supplied) Prolonged use of glucocorticoids can lead to the formation of a cataract, however the mechanism is not known. We recently reported the presence of the functional glucocorticoid receptor in immortalized cultured mammalian lens epithelial cells (Gupta & Wagner, Invest Ophthalmol Vis Sci 2003), but the short term biological effect of glucocorticoid action in lens epithelial cells is not known. This study seeks to examine glucocorticoid induced changes in global gene expression in LECs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1353

Platform:

GPL96

12 Samples

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Analysis of cultured immortalized lens epithelial cells (LEC) 4 and 16 hours after treatment with 1 uM dexamethasone, a glucocorticoid steroid hormone. Long-term glucocorticoid use can induce cataract formation. Results identify glucocorticoid targets and short-term effects of glucocorticoid on LECs

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL96

Series:

GSE3040

12 Samples

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(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) Primary open angle glaucoma (POAG) is a progressive optic neuropathy, which is a major cause of worldwide visual impairment and blindness. Pathological hallmarks of the glaucomatous optic nerve head include retinal ganglion cell axon loss and extracellular matrix (ECM) remodeling of the lamina cribrosa layer. TGF-beta is an important pro-fibrotic modulator of ECM metabolism, whose levels are elevated in human POAG lamina cribrosa tissue compared with non-glaucomatous controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1313

Platform:

GPL96

4 Samples

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Analysis of glial fibrillary acidic acid (GFAP)-negative lamina cribrosa (LC) glial cell response to 10 ng/ml TGF-beta1 for 24 hours. This is a model for primary open-angle glaucoma (POAG) in which TGF-beta levels are elevated in human LC tissue.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL96

Series:

GSE2705

4 Samples

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(Submitter supplied) Olfactory bulb transcript levels for GFAP transgenic mice compared to wildtype at 3wks and 4mos Keywords = GFAP Keywords = Rosenthal fibers Keywords = Alexander disease Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1488

Platform:

GPL81

12 Samples

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Analysis of olfactory bulb of 23 day and 4 month old transgenics overexpressing human intermediate filament protein GFAP. GFAP missense mutations result in Alexander disease, a neurodegenerative disease. GFAP overexpression results in an encephalopathy similar to that resulting from GFAP mutations.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age, 2 genotype/variation sets

Platform:

GPL81

Series:

GSE977

12 Samples

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(Submitter supplied) Total RNA was isolated from candoxin-treated (n = 3 flasks of each time points - 12hrs, 24hrs and 48hrs) and untreated control (n = 3) cells using RNeasy® mini kit.The preparation and processing of labeled, fragmented cRNA for oligonucleotide mcroarray hybridization has been performed according to the manufacturer’s protocols described in the technical manual (Affymetrix, Santa Clara, CA). Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1414

Platform:

GPL96

12 Samples

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Analysis of glial cell line Hs683 at various time points up to 48 hours following exposure to 300 nM of candoxin (CDX) snake venom. CDX is a neurotoxin that inhibits post-synaptic neuromuscular and neuronal alpha7nACh-receptors, and delays cell death in glial cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL96

Series:

GSE1682

12 Samples

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(Submitter supplied) The mechanical effect of raised intraocular pressure is a recognised stimulus for optic neuropathy in primary open angle glaucoma (POAG). Characteristic extra-cellular matrix (ECM) remodelling accompanies axonal damage in the lamina cribrosa (LC) of the optic nerve head in POAG. Glial cells in the lamina cribrosa may play a role in this process but the precise cellular responses to mechanical forces in this region are unknown. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

2 Samples

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(Submitter supplied) To evaluate gene expression in human peripheral blood derived monocytes over the course of an LPS stimulation time-series. Keywords: time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2856

Platform:

GPL570

10 Samples

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Analysis of peripheral blood-derived monocytes at various time points up to 24 hours following treatment with lipopolysaccharide. Results compared with those obtained from Massively Parallel Signature Sequencing.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 5 time sets

Platform:

GPL570

Series:

GSE5504

10 Samples

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(Submitter supplied) Phenotypic and functional diversity between macrophage subpopulations reflects their plasticity to respond to microenvironmental signals. Apart from detecting differences in expression profiles, the comparison of the transcriptomes of different macrophage populations may also allow the definition of molecular similarities between these subsets. Transcriptome analysis of human Kuppfer cells, alveolar, splenic and atherosclerotic plaque residing macrophages using microarrays, identified 42 genes that are specifically expressed in atherosclerotic plaque macrophages. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4868

30 Samples

Download data: TXT

(Submitter supplied) We compare, in human macrophages, stimuli considered to induce M1 or M2 type of activation, also termed classical and alternative activation. This dataset highlights key differences between the M1 and M2 stimuli pointing to the need of a revision of the paradigm. Macrophages were left untreated or stimulated with IL-4 and IL-13 (prototypic M1 stimuli), Dexamethasone (Glucocorticoid receptor agonist) which has been hitherto considered M2, IL-10 which also falls under the M2 umbrella, and LPS and Interferon Gamma considered M1 stimuli. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

7 Samples

Download data: TXT

(Submitter supplied) The human AA dataset 5 includes the gene expression profile of monocyte-derived macrophages isolated from blood donor buffy coats and plated on TCP in medium containing 10% autologous serum. Cells were stimulated for 120 hours with 50 ng/ml of IL-4, 50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α, 50 ng/ml of M-CSF or 50 ng/ml of IL-10. RNA samples were isolated using TriPure isolation reagent (Roche) and the RNeasy Mini Kit-Qiagen Clean up method. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

5 related Platforms

73 Samples

Download data: CEL

(Submitter supplied) Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from bone marrow derived macrophages upon exposure to 20 ng/ml of IL-4 for 18 hours.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL