GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE3040 Query DataSets for GSE3040
Status Public on Nov 14, 2005
Title Glucocorticoid Induced Changes in Gene Expression in Human Lens Epithelial Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Prolonged use of glucocorticoids can lead to the formation of a cataract, however the mechanism is not known. We recently reported the presence of the functional glucocorticoid receptor in immortalized cultured mammalian lens epithelial cells (Gupta & Wagner, Invest Ophthalmol Vis Sci 2003), but the short term biological effect of glucocorticoid action in lens epithelial cells is not known. This study seeks to examine glucocorticoid induced changes in global gene expression in LECs. RNA isolated from HLE B-3 cells, treated with Dex or Veh for 4 or 16 hours, was used to analyze global changes in gene expression by microarray hybridization. Microarray studies at 2 time periods demonstrate that glucocorticoids modulate gene expression in immortalized human LECs, reveal novel changes in gene expression, and confirms an endogenous genomic lens glucocorticoid response.
Keywords: treatment response
Overall design HLE B-3 cells were treated with 1uM dexamethasone or vehicle for 4 or 16 hours in triplicate. Total RNA was converted to cRNA and hybridized to Affymetrix HUM_133A genechips. Images were acquired using the Affymetrix GeneArray scanner. Data was extracted using Affymetrix Microarray Suite 5.0.
Contributor(s) Gupta VK, Galante A, Soteropoulos P, Wagner BJ
Citation(s) 16319822
Submission date Aug 01, 2005
Last update date Aug 10, 2018
Contact name Vanita Gupta
Organization name UMDNJ
Department Biochemistry & Molecular Biology
Street address 185 South Orange Ave
City Newark
State/province NJ
ZIP/Postal code 07103
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (12)
GSM66605 Vehicle-4 hour 1
GSM66606 Vehicle-4 hour 2
GSM66607 Vehicle-4 hour 3
BioProject PRJNA93055

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap