GEO DataSets

Analysis of normal fibroblasts and endothelial cells, at various population doubling levels (PDLs), following human telomerase reverse transcriptase (hTERT) transfection. hTERT mediates cellular immortalization. Results identify cell-type specific transcriptional responses during immortalization.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 cell type, 5 time sets

Platform:

GPL1530

Series:

GSE1900

12 Samples

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(Submitter supplied) A normal neonatal human diploid fibroblast strain MJ90 was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Sigma-Aldrich Japan) supplemented with 2 mM L-glutamine (Life Technologies) and 10% fetal calf serum (FCS; Life Technologies). A normal human umbilical vein endothelial cell strain HUE142-2 were grown in MCDB151 medium (Sigma-Aldrich Japan) supplemented with 15% fetal bovine serum, 5 µg/ml of heparin (Sigma), and 5 ng/ml of recombinant acidic fibroblast growth factor in a plastic flask (Corning) precoated with bovine fibronectin solution (1 µg/cm2, Wako).The 40 µg of plasmid hTRT/FLAG or hTERTn2 (from Prof. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1760

Platform:

GPL1530

12 Samples

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(Submitter supplied) Activation of telomerase often endows cancer cells, but rarely normal somatic cells, with immortality. Especially, fetal lung fibroblasts are known to be hardly immortalized by TERT overexpression. We here established an immortal non-transformed lung fibroblast cell line only by TERT transfection, as well as an immortal transformed cell line by transfection of TERT and SV40 early antigens. Comparing the expression profiles of these cell lines with those of mortal cell strains with elongated lifespan after TERT transfection, 51 genes, including 19 upregulated and 32 downregulated, were explored to be the candidates responsible for regulation of cellular proliferation of lung fibroblasts. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL1928 GPL570 GPL4133

17 Samples

Download data: CEL, TXT

(Submitter supplied) Gene expression profile of Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) reveals murine targets for preclinical development of human prostate cancer therapy In this study, we have generated an open source TRAMP microarray dataset to identify differentially expressed genes from human prostate cancer that have concordant expression in TRAMP tumors, and thereby represent lead targets for preclinical therapy development. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

18 Samples

Download data: CEL

(Submitter supplied) We used high density oligonucleotide arrays to measure the relative expression levels of ~27,000 probe sets in the left ventricles of inbred rat strains; DA (high performing), Copenhagen (COP, low performing), as well as F1(COPxDA) rats bred from these two strains whose performance was intermediate relative to both parental strains. DA and COP rats differ for both intrinsic aerobic running capacity (assessed with a treadmill exercise test to exhaustion) and intrinsic cardiac performance (as assessed by the Langendorff-Neeley working heart model). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS778 GDS779 GDS780

Platforms:

GPL86 GPL87 GPL85

36 Samples

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Expression profiling of heart left ventricles from 15 week old inbred COP, DA, and COP x DA F1 offspring (low, high, and intermediate performers respectively). Results provide insight into mechanisms underlying aerobic capacity.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 strain sets

Platform:

GPL87

Series:

GSE1795

12 Samples

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Expression profiling of heart left ventricles from 15 week old inbred COP, DA, and COP x DA F1 offspring (low, high, and intermediate performers respectively). Results provide insight into mechanisms underlying aerobic capacity.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 strain sets

Platform:

GPL86

Series:

GSE1795

12 Samples

Download data

Expression profiling of heart left ventricles from 15 week old inbred COP, DA, and COP x DA F1 offspring (low, high, and intermediate performers respectively). Results provide insight into mechanisms underlying aerobic capacity.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 strain sets

Platform:

GPL85

Series:

GSE1795

12 Samples

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(Submitter supplied) Lentivirus containing simian virus 40 large T antigen (SV40T) is routinely used to induce cell immortalization. However, the roles of viral integration itself in this progress is still controversial. Here, we transformed primary mouse embryonic fibroblasts (MEFs) with SV40T lentivirus and studied the roles of viral integration in the immortalization using RNA-seq and whole genome sequencing (WGS). During the immortalization, differentially expressed genes (DGEs) are enriched in viral infection and several diverse activities. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21273

18 Samples

Download data: XLSX