GEO DataSets

Expression profiling of astrocytes taken from the optic nerve head (ONH) of patients with glaucoma. The ONH is the site of damage in glaucomatous optic neuropathy. Studies suggest that damage in glaucoma is mediated by reactive astrocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL8300

Series:

GSE2378

13 Samples

Download data: CEL, EXP

(Submitter supplied) Astrocytes from optic nerve head from donors with and without glaucoma Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1112

Platforms:

GPL8300 GPL91

15 Samples

Download data: CEL, EXP

(Submitter supplied) Gene expression in human optic nerve head (ONH) astrocytes exposed to either 60 mm Hg hydrostatic pressure (HP) or control ambient pressure (CP) was compared using Affymetrix GeneChip microarrays to identify HP-responsive genes. Primary ONH astrocytes from two male Caucasian donors (passage 4) were grown to 75% confluence and were exposed for 6, 24 or 48 h to control ambient pressure (CP6, CP24, CP48) or hydrostatic pressure (HP6, HP24, HP48), or harvested at the beginning of the pressure experiment (CP0). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS532

Platform:

GPL8300

44 Samples

Download data

Temporal analysis of how astrocytes in the optic nerve head (ONH) respond to changes in intraocular pressure (IOP). ONH astrocytes exposed to 60 mm Hg hydrostatic pressure for 6, 24, and 48 hours. Lends understanding to pathogenesis of glaucoma.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol, 4 time sets

Platform:

GPL8300

Series:

GSE758

44 Samples

Download data

(Submitter supplied) The mechanical effect of raised intraocular pressure is a recognised stimulus for optic neuropathy in primary open angle glaucoma (POAG). Characteristic extra-cellular matrix (ECM) remodelling accompanies axonal damage in the lamina cribrosa (LC) of the optic nerve head in POAG. Glial cells in the lamina cribrosa may play a role in this process but the precise cellular responses to mechanical forces in this region are unknown. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

2 Samples

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(Submitter supplied) Primary open angle glaucoma (POAG) is a progressive optic neuropathy, which is a major cause of worldwide visual impairment and blindness. Pathological hallmarks of the glaucomatous optic nerve head include retinal ganglion cell axon loss and extracellular matrix (ECM) remodeling of the lamina cribrosa layer. TGF-beta is an important pro-fibrotic modulator of ECM metabolism, whose levels are elevated in human POAG lamina cribrosa tissue compared with non-glaucomatous controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1313

Platform:

GPL96

4 Samples

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Analysis of glial fibrillary acidic acid (GFAP)-negative lamina cribrosa (LC) glial cell response to 10 ng/ml TGF-beta1 for 24 hours. This is a model for primary open-angle glaucoma (POAG) in which TGF-beta levels are elevated in human LC tissue.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL96

Series:

GSE2705

4 Samples

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(Submitter supplied) Purpose: Marked extracellular matrix (ECM) remodeling occurs in the human optic nerve head in primary open angle glaucoma (POAG). The glial fibrillary acid protein (GFAP) negative lamina cribrosa cell may play an important role in this remodeling process. The authors report the first study of global and ECM-focused gene transcription differentials between GFAP-negative negative lamina cribrosa (LC) cells from normal and POAG human donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

4 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

81 Samples

Download data: CEL

(Submitter supplied) To determine whether optic nerve head astrocytes, a key cellular component of glaucomatous neuropathy, exhibit differential gene expression in primary culture of astrocytes from African American donors with or without glaucoma, compared to astrocytes from Caucasian American donors with or without glaucoma. Keywords: Gene expression profile

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL571 GPL8300 GPL91

63 Samples

Download data: CEL

(Submitter supplied) To obtain and analyze early retinal changes at molecular level at 24h after ipsilateral intraorbital nerve radiation injury by gamma knife surgery

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

6 Samples

Download data: CEL

(Submitter supplied) Glaucoma is a common ocular disorder that is a leading cause of blindness worldwide. It is characterized by the dysfunction and loss of retinal ganglion cells (RGCs). Although many studies have implicated various molecules in glaucoma, no mechanism has been shown to be responsible for the earliest detectable damage to RGCs and their axons in the optic nerve. Here, we show that the leukocyte transendothelial migration pathway is activated in the optic nerve head at the earliest stages of disease in an inherited mouse model of glaucoma. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL1261

49 Samples

Download data: CEL, TXT

(Submitter supplied) Glaucoma, a multifactorial neurodegenerative disease characterized by progressive loss of retinal ganglion cells and their axons in the optic nerve, is a leading cause of irreversible vision loss. Intraocular pressure (IOP) is a risk factor for axonal damage, which initially occurs at the optic nerve head (ONH). Complex cellular and molecular mechanisms involved in the pathogenesis of glaucomatous optic neuropathy remain unclear. more...

Organism:

Felis catus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17081

16 Samples

Download data: GTF, TXT

(Submitter supplied) A major risk factor for glaucomatous optic neuropathy is the level of intraocular pressure (IOP), which can lead to retinal ganglion cell axon injury and cell death. The optic nerve has a rostral unmyelinated portion at the optic nerve head followed by a caudal myelinated region. The unmyelinated region is differentially susceptible to IOP-induced damage in rodent models and in human glaucoma. While several studies have analyzed gene expression changes in the mouse optic nerve following optic nerve injury, few were designed to consider the regional gene expression differences that exist between these distinct areas. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

36 Samples

Download data: CSV, TSV

(Submitter supplied) Vascular hypoperfusion is a pathological phenomenon in the glaucomatous optic nerve head. We report transcriptional responses in GFAP-negative LC cells exposed to in-vitro hypoxic stress (1%O2).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

6 Samples

Download data: CEL

(Submitter supplied) Global genome-wide sequencing analysis of RNA prepared from Cyp1b1-/-AC and Cyp1b1+/+AC was perfomed to gain additional insight into the cellular regulatory mechanisms impacted by Cyp1b1 expression

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: XLSX

(Submitter supplied) Primary cultures of astrocytes from rat optic nerve heads were treated with EGFR ligand, EGF. Two cell lines from two different rat donors were used. The sister cell cultures were set as control and EGF treated groups. Keywords: rat optic nerve head astrocytes

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2146

Platform:

GPL1355

12 Samples

Download data: CEL

Analysis of astrocytes 4 or 12 hours after epidermal growth factor (EGF) treatment to activate the EGF receptor (EGFR). EGFR is activated in astrocytes after various injuries to optic nerves. Results provide insight into the role of EGFR in the formation of reactive astrocytes after neural injury.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL1355

Series:

GSE5282

12 Samples

Download data: CEL

(Submitter supplied) The total RNA from control and POAG optic nerve lamina cribrosa region was isolated and subjected to analysis on U133A 2.0 affymetrix microarray chip. The results from these two analysis was compared to determine whether PAD II RNA level is altered. Purpose of the study. This study was done to determine which RNA transcripts undergo significant changes in the optic nerve between normal and cadaver primary open-angle glaucoma donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

2 Samples

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(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL