GEO DataSets

(Submitter supplied) Bone marrow plasma cells (BMPCs) of varying lifespans are generated through a germinal center response (GC). The developmental dynamics and genomic programs of antigen-specific plasma cell (PC) precursors remain to be elucidated. Using a model antigen, we demonstrate biphasic generation of BMPC precursors, with those generating long-lived PCs preferentially produced in late phase of GC response. Clonal tracing using scRNA-seq+BCR-seq in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveal a novel PC transition state that gives rise to functionally competent PC precursors, the latter undergo clonal expansion, dependent on inducible expression of TIGIT. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: H5, TAR