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| Status |
Public on Feb 01, 2024 |
| Title |
Temporal dynamics and genomic programming of plasma cell fates |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bone marrow plasma cells (BMPCs) of varying lifespans are generated through a germinal center response (GC). The developmental dynamics and genomic programs of antigen-specific plasma cell (PC) precursors remain to be elucidated. Using a model antigen, we demonstrate biphasic generation of BMPC precursors, with those generating long-lived PCs preferentially produced in late phase of GC response. Clonal tracing using scRNA-seq+BCR-seq in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveal a novel PC transition state that gives rise to functionally competent PC precursors, the latter undergo clonal expansion, dependent on inducible expression of TIGIT. We propose a model for the proliferation and programming of precursors of long-lived BMPCs, based on extended antigen encounters.
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| Overall design |
Splenic and bone marrow CD138+ cells or subsets were isolated using positive selection or flow cytometry based sorting from C57BL6/J mice immunized with NP-KLH, alum and LPS at indicated days post-immunization (d.p.i.)
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| Web link |
https://www.nature.com/articles/s41590-024-01831-y
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| Contributor(s) |
Manakkat Vijay GK, Singh H |
| Citation(s) |
38698087 |
| Submission date |
Aug 14, 2023 |
| Last update date |
May 06, 2024 |
| Contact name |
Harinder Singh |
| E-mail(s) |
harinder@pitt.edu
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| Phone |
6503034820
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| Organization name |
University of Pittsburgh
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| Department |
Center for Systems Immunology and Department of Immunology
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| Lab |
Dr Harinder Singh
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| Street address |
5051 Centre Avenue
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| GSM7709781 |
Spleen, 21 d.p.i., CD138+, BCR-seq |
| GSM7709782 |
Spleen, 14 d.p.i., NP-specific B cells, scRNA-seq |
| GSM7709783 |
Spleen, 14 d.p.i., NP-specific B cells, BCR-seq |
| GSM7709784 |
Spleen, 35 d.p.i., B220-CD138+, scRNA-seq |
| GSM7709785 |
Spleen, 35 d.p.i., B220-CD138+, BCR-seq |
| GSM7709786 |
Spleen, 35 d.p.i., B220-B220intCD138+, scRNA-seq |
| GSM7709787 |
Spleen, 35 d.p.i., B220-B220intCD138+, BCR-seq |
| GSM7709788 |
Bone-marrow, 35 d.p.i., B220-B220intCD138+, scRNA-seq |
| GSM7709789 |
Bone-marrow, 35 d.p.i., B220-B220intCD138+, BCR-seq |
| GSM7709790 |
Spleen, 35 d.p.i., B220-B220intB220+CD138+, scRNA-seq |
| GSM7709791 |
Spleen, 35 d.p.i., B220-B220intB220+CD138+, BCR-seq |
| GSM7709792 |
Bone-marrow, 35 d.p.i., B220-B220intB220+CD138+, scRNA-seq |
| GSM7709793 |
Bone-marrow, 35 d.p.i., B220-B220intB220+CD138+, BCR-seq |
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| Relations |
| BioProject |
PRJNA1005291 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE240813_RAW.tar |
196.3 Mb |
(http)(custom) |
TAR (of H5, TAR) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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