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GEO DataSets

Functional Genomics Studies

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Items: 4

Select item 2000451151.

Expression profiling of 376 wildtypes to assess day-to-day variance

(Submitter supplied) A collection of budding yeast wild types grown in parallel to 1,484 budding yeast deletion mutants to assess day-to-day variance. Related to series GSE42526 and GSE42527.

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL11232
755 Samples

Download data: TXT

Series

Accession:: GSE45115

ID:: 200045115

Select item 2000256442.

Expression profiling of yeast kinase and phosphatase knockouts

(Submitter supplied) To understand relationships between phosphorylation-based signaling pathways, we analyzed 150 deletion mutants of protein kinases and phosphatases in S. cerevisiae using DNA microarrays. Downstream changes in gene expression were treated as a phenotypic readout. Double mutants with synthetic genetic interactions were included to investigate genetic buffering relationships such as redundancy. Three types of genetic buffering relationships are identified: mixed epistasis, complete redundancy and quantitative redundancy. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL11232
464 Samples

Download data: TXT

Series

Accession:: GSE25644

ID:: 200025644

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000112323.

A-UMCU-Y16k-1.3

(Submitter supplied) 1. Oligos are resuspended and adjusted to 10µM in 150mM phosphatebuffer, pH8.5 in Genetix 384-wells plates 2. Array production is done using a Biorobotics MicroGridII spotter located in a low dust cleanroom (ISO7/ downflow ISO5) at 18C, at 48% humidity 3. Slide postprocessing is carried out according to CodeLink protocol: Printed slides are kept at 20C, 75% humidity for 24 hours 4. Residual reactive groups are blocked using pre-warmed blocking solution (50 mM ethanolamine, 0.1 M Tris (pH 9) at 50C for 30 min; slides are rinsed thoroughly with milliQ water; slides are washed with 4x SSC, 0.1% SDS (pre-warmed to 50C) for 30 min at 50C on a shaker 5. more...