GEO DataSets

(Submitter supplied) Cell lines. HEK293 human embryonic kidney cells were obtained from ATCC (American Type Culture Collection, Manassas, VA) and were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Heat inactivated, Hyclone, Logan, Utah) and 1X antibiotics/glutamine (100 units/ml penicillin, 100ug/ml streptomycin, 292ug/ml L-Glutamine sulfate, all from Invitrogen Corp, Carlsbad, CA.), under either hypoxic (1% O2) or normoxic (21% O2) conditions at 37C in a tissue culture incubator. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2018

Platform:

GPL1528

10 Samples

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Analysis of HEK293T embryonic kidney cells subjected to hypoxia or overexpressing hypoxia-inducible factor (HIF)-1, degradation resistant HIF-1, or HIF-2. Results provide insight into the respective roles of HIF-1 and HIF-2 in the cellular response to hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 protocol sets

Platform:

GPL1528

Series:

GSE2020

10 Samples

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(Submitter supplied) Attachment: UV cross-linked Array description: The custom arrays are manufactured by the NCI Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC). Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com). Protocol: The human long oligonuleotide probe set contains 21,329 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/ ) . more...

Organism:

Homo sapiens

2 DataSets

16 Series

1045 Samples

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