GEO DataSets

(Submitter supplied) DNA methylation occurs at preferred sites in eukaryotes, although the basis for preference is not known. We use a microarray-based profiling method to explore the involvement of Arabidopsis CMT3 and DRM DNA methyltransferases, a histone H3 lysine-9 methyltransferase (KYP) and an Argonaute-related RNA silencing component (AGO4) in methylating target loci. We find that KYP targets are also CMT3 targets, suggesting that histone methylation maintains CNG methylation genome-wide. more...

Organism:

Arabidopsis thaliana

Type:

Methylation profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL1911 GPL2726

60 Samples

Download data

(Submitter supplied) To obtain the 240 random single-copy loci, nonoverlapping 1-kb segments were selected at random from a database consisting of available contigs for A. thaliana chromosomes 1, 3, and 5 and complete chromosomes 2 and 4 from GenBank (August 2, 2000). The Primer3 program running on a SUN Solaris workstation was applied with default settings to select 17-bp candidate primers with Tm > 50°C that encompass 700 ± 25 bp fragments. more...

Organism:

Arabidopsis thaliana

1 Series

30 Samples

Download data