GEO DataSets

(Submitter supplied) Since initial regulatory action in 2010 in Canada, bisphenol A (BPA) has been progressively replaced by structurally related alternative chemicals. Unfortunately, many of these chemicals are data-poor, limiting toxicological risk assessment. We used high-throughput transcriptomics to evaluate potential hazards and compare potencies of BPA and 15 BPA alternative chemicals in cultured breast cancer cells. more...

Organism:

Homo sapiens; synthetic construct

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18573 GPL19424

768 Samples

Download data: CSV

(Submitter supplied) To assess whether genes associated with BH4Ds are more or less sensitive than genes associated with sharp H3K4me3 peaks to perturbation of H3K4me3 levels, we performed RNA-Seq experiment from Jurkat cells treated with DMSO or with 2-(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT), a specific inhibitor of JARID1 family of H3K4me3 demethylases, also known as KDM5 (Blair et al. 2011; Sayegh et al. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

3 Samples

Download data: CSV

(Submitter supplied) To assess whether BH4Ds are more or less sensitive than sharp H3K4me3 peaks to perturbation of H3K4me3 levels, we performed H3K4me3 ChIP-seq experiments from Jurkat cells treated with DMSO or with either 2-(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT), a specific inhibitor of JARID1 family of H3K4me3 demethylases, also known as KDM5, or OICR-9429, an inhibitor of the MLL-WDR5, previously shown to decrease cellular levels of H3K4me3.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL18573

4 Samples

Download data: BW

(Submitter supplied) High throughput screening and subsequent hit validation identified 4-isopropyl-3-(2-((1-phenylethyl) amino)pyrimidin-4-yl)oxazolidin-2-one as a potent inhibitor of IDH1R132H. Synthesis of the 4 separate diastereomers identified the (S,S)-diastereomer (IDH125) as the most potent isomer. This also showed reasonable cellular activity and excellent selectivity vs IDH1wt. Initial SAR exploration identified the key tolerances and potential for optimization. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

39 Samples

Download data: IDAT, TXT

(Submitter supplied) The nematode Caenorhabditis elegans offers currently untapped potential for carrying out high-throughput, live-animal screens of low molecular weight compound libraries to identify molecules that target a variety of cellular processes. We previously used a bacterial infection assay in C. elegans to identify 119 compounds that affect host-microbe interactions among 37,214 tested. We subsequently found that one of these small molecules, RPW-24, protects C. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS4243

Platform:

GPL200

6 Samples

Download data: CEL

Analysis of bacterial infected C. elegans treated with 70 μM RPW-24 or DMSO. In a C.elegans-bacterial infection assay screening small molecules, RPW-24 was identified as a putative antimicrobial agent. Results provide insight into C. elegans conserved immune response to bacterial infection.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL200

Series:

GSE37266

6 Samples

Download data: CEL