GEO DataSets

(Submitter supplied) Aspergillus fumigatus transcription factor AtrR is a critical determinant of the azole resistance phenotype of this organism. AtrR positively regulates expression of a range of genes involved in azole resistance including the ATP-binding cassette transporter-encoding locus abcG1 (cdr1B/abcC) as well as the gene that encodes the enzymatic target of azole drugs cyp51A. Homology searches of A. fumigatus AtrR against a range of fungal species identified highly conserved 25 amino acid region in the carboxy-terminus. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23160

21 Samples

Download data: BIGWIG, XLSX

(Submitter supplied) The RNA interference (RNAi) pathway has evolved numerous functionalities in eukaryotes, with many on display in Kingdom Fungi. RNAi can regulate gene expression, facilitate drug resistance, or even be altogether lost to improve virulence potential in some fungal pathogens. In the WHO fungal priority pathogen, Aspergillus fumigatus, the RNAi system is known to be intact and functional. To extend our limited understanding of A. more...

Organism:

Aspergillus fumigatus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL33056

36 Samples

Download data: CSV

(Submitter supplied) Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the tRNA fragment and tRNA half repertoire of A. fumigatus in wild-type conidia and mycelium grown for 24 or 48 hours in liquid culture.

Organism:

Aspergillus fumigatus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23268

9 Samples

Download data: CSV

(Submitter supplied) Pathogens have to cope with oxidative, iron- and carbon-limitation stresses in the human body. To understand how combined iron-carbon limitation alters oxidative stress response, Aspergillus fumigatus was cultured in glucose-peptone or peptone containing media supplemented or not with deferiprone as iron chelator. Changes in the transcriptome in these cultures were recorded after H2O2 treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

24 Samples

Download data: XLSX

(Submitter supplied) We generated this RNA-seq dataset to investigate the effects of transcription factor ZfpA (aka. AFUA_8G05010 or AFUB_082490) in the gene expression of A. fumigatus strain Af293. One of the major reasons we were interested in investigating ZfpA was because this TF had been identified as highly regulated by the oxylipins (https://pubmed.ncbi.nlm.nih.gov/33056992/). To that end, we collected RNA from spores and the mycelial tissue under three distinct conditions: (a) wild-type ZfpA, (b) ZfpA knocked out, and (c) ZfpA overexpressed. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33056

24 Samples

Download data: CSV, TSV

(Submitter supplied) Increasing antifungal drug resistance is a major concern associated with human fungal infections. Genetic mutation and epimutation mechanisms are clearly linked to resistance in some fungi, but few studies have investigated RNA modifications (the epitranscriptome) as a mediator of resistance. Here, deletion of the Aspergillus fumigatus tRNA-modifying isopentenyl transferase ortholog, Mod5, led to altered growth and stress response, but also unexpected resistance against the antifungal drug 5-fluorocytosine (5-FC). more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33056

12 Samples

Download data: XLSX

(Submitter supplied) The antifungal compound fludioxonil targets the fungal specific group III hybrid histidin kinaseTcsC of A. fumigatus, which in turn activate the High Osmolarity Glycerol (HOG) pathway and leads to drastic swelling of the fungal cells. We investigated the different responses of the AfS35 (wild type), the completely resistant mutant strain DtcsC and the partial resistant mutant strain Dskn7 to fludioxonil.

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

36 Samples

Download data: XLS

(Submitter supplied) This study aimed to understand the differences in gene expression when comparing Aspergillus fumigatus swollen conidia that overexpress an artifical Bir1 construct (strain published in Shlezinger et. al 2017) versus a parental control strain in RPMI + 0.5ug/mL voriconazole. The purpose of this experiment was to understand what basal expression differences may drive a decreased susceptibility to hydrogen peroxide upon subsequent treatment (not tested in this submission). more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

12 Samples

Download data: CSV, XLSX

(Submitter supplied) To determine the genes responsive to FfmA in A. fumigatus, a doxycycline-repressible (dox off) gene fusion to the ffmA coding sequence was constructed. This strain was designated SPF182. These cells were grown for 16 hours in the absence of doxycycline and then shifted into medium containing doxycycline (+dox) or untreated (-dox). After 8 additional hours, the cells were harvested and total RNA prepared. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23160

9 Samples

Download data: BIGWIG

(Submitter supplied) Two strains were analyzed by ChIP-seq to determine the genomic binding sites for the Aspergillus fumigatus transcription factor FfmA. A wild-type strain (AfS35) was subjected to ChIP-seq analysis using an antibody directed against the wild-type version of the FfmA protein. An strain containing an epitope-tagged version (FLAG-ffmA) of the ffmA gene under control of a doxycycline off (dox off) promoter was also analyzed by ChIP-seq. more...

Organism:

Aspergillus fumigatus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

8 Samples

Download data: BIGWIG

(Submitter supplied) Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the small RNA repertoire of A. fumigatus in conidia and mycelium grown for 24 or 48 hours in liquid culture.

Organism:

Aspergillus fumigatus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28001

9 Samples

Download data: XLSX

(Submitter supplied) The RNA interference (RNAi) pathway has evolved numerous functionalities in eukaryotes, with many on display in Kingdom Fungi. RNAi can regulate gene expression, facilitate drug resistance, or even be altogether lost to improve virulence potential in some fungal pathogens. In the WHO fungal priority pathogen, Aspergillus fumigatus, the RNAi system is known to be intact and functional. To extend our limited understanding of A. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33056

36 Samples

Download data: XLSX

(Submitter supplied) Glucose is a widely used carbon source in laboratory practice to culture Aspergillus fumigatus, however, glucose availability is often low in its “natural habitats” including the human body. We used a physiological–transcriptomical approach to reveal differences between A. fumigatus Af293 cultures incubated on glucose, glucose and peptone, peptone (carbon limitation), or without any carbon source (carbon starvation). more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

12 Samples

Download data: XLSX

(Submitter supplied) Genome sequence data results are reported from experimental and bioinfomatic work using the technique 'Bulk Segregant Analysis' to determine the genetic basis of observed resistance to the azole antifungal compound itraconazole in the opportunistic fungal pathogen Aspergillus fumigatus.

Organism:

Aspergillus fumigatus

Type:

Genome variation profiling by high throughput sequencing

Platform:

GPL31245

18 Samples

Download data: VCF

(Submitter supplied) Transcriptome analysis of deletion mutant which coding histone modification enzymes compared to wild type by NGS

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

18 Samples

Download data: TXT

(Submitter supplied) Transcriptomic analysis of Aspergillus fumigatus and Klebsiella pneumoniae grown as single and mixed biofilms.

Organism:

Klebsiella pneumoniae; Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23160 GPL25164 GPL32070

9 Samples

Download data: XLSX

(Submitter supplied) Aspergillus fumigatus is a human-pathogenic mold that extracts nutrients from the environment or from host tissues by secreting hydrolytic enzymes. The ability of A. fumigatus to adjust secretion levels in proportion to demand relies on the assistance of the unfolded protein response (UPR), an adaptive stress response pathway that regulates the unique protein-folding environment of the endoplasmic reticulum (ER). more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30331

6 Samples

Download data: XLSX

(Submitter supplied) Invasive Pulmonary Aspergillosis (IPA) is a life-threatening fungal infection especially in immunocompromised population. The low availability of antifungal drugs coupled with the emergence of antifungal resistance has become an important clinical concern. Caspofungin (CSP) is recommended as second-line therapy but resistance and tolerance mechanisms have been reported. However, how the fungal cell articulates the response to CSP is not completely understood. more...

Organism:

Aspergillus fumigatus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

12 Samples

Download data: BW

(Submitter supplied) Transcriptome analysis of deletion mutant which coding HAT and APSES TF compared to wild type by NGS

Organism:

Aspergillus fumigatus Af293

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30552

15 Samples

Download data: TXT

(Submitter supplied) By searching for new drugs against fungal pathogens, we found that miltefosine is active against Aspergillus fumigatus clinical isolates. A library of transcription factors (TF) null mutants was then challenged with this drug and we discovered a novel TF that confers resistance to miltefonise, named here SmiA. By using ChIP-seq, we searched for SmiA targets upon miltefosine treatment.

Organism:

Aspergillus fumigatus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

4 Samples

Download data: BW