|
| Status |
Public on May 03, 2009 |
| Title |
Keratinocytes_Erk1andErk2siRNA-A_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary human keratinocytes treated with siRNA against Erk1 and Erk2 (oligo pair A)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary human foreskin keratinocytes
|
| Biomaterial provider |
Stanford University
|
| Growth protocol |
Keratinocyte SFM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy
|
| Label |
Biotin
|
| Label protocol |
Performed by Stanford PAN Facility
|
| |
|
| Hybridization protocol |
Performed by Stanford PAN Facility
|
| Scan protocol |
Performed by Stanford PAN Facility
|
| Description |
Primary human keratinocytes were grown in vitro and treated with either a scrambled control siRNA, a pair of siRNA oligomers against Erk1 and Erk2, or an independent pair of siRNA oligomers against Erk1 and Erk2. RNA was harvested four days later. Three biological replicates were performed for each of the three groups. See PA Dumesic et al. 2009 Journal of Cell Biology for experimental details.
|
| Data processing |
RMA preprocessing normalization by GeneSpring 7.3.
|
| |
|
| Submission date |
Mar 26, 2009 |
| Last update date |
Apr 20, 2009 |
| Contact name |
Phillip Anthony Dumesic |
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
360 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE15417 |
Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes |
|
