|
| Status |
Public on May 03, 2009 |
| Title |
Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We assessed the effect of RNAi-mediated MAP kinase cascade signaling blockade in primary human keratinocytes. Two sets of siRNA targeting different regions of the Erk1/2 genes were used, enabling identification of off-target siRNA effects.
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| |
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| Overall design |
Primary human keratinocytes were electroporated with scrambled control siRNA, a pair of siRNA oligomers against Erk1 and Erk2 (set A), or an independent pair of siRNA oligomers against Erk1 and Erk2 (set B). Four days after electroporation, RNA was harvested. Three biological replicates were performed for each of the three siRNA groups.
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| |
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| Contributor(s) |
Dumesic PA, Scholl FA, Barragan DI, Khavari PA |
| Citation(s) |
19414607 |
| Submission date |
Mar 26, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Phillip Anthony Dumesic |
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
360 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (9)
|
| GSM386971 |
Keratinocytes_Erk1andErk2siRNA-A_rep1 |
| GSM386972 |
Keratinocytes_Erk1andErk2siRNA-A_rep2 |
| GSM386973 |
Keratinocytes_Erk1andErk2siRNA-A_rep3 |
| GSM386975 |
Keratinocytes_Erk1andErk2siRNA-B_rep1 |
| GSM386976 |
Keratinocytes_Erk1andErk2siRNA-B_rep2 |
| GSM386977 |
Keratinocytes_Erk1andErk2siRNA-B_rep3 |
|
| Relations |
| BioProject |
PRJNA115823 |
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