|
| Status |
Public on May 28, 2009 |
| Title |
MCF-7 cells_NAMPT knockdown_replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 cells NAMPT knockdown
|
| Organism |
Homo sapiens |
| Characteristics |
MCF-7 cells NAMPT knockdown
|
| Growth protocol |
MCF-7 cells were maintained in Eagle's minimum essential medium supplemented with 5% calf serum and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Trizol reagent according to the manufacturer's instructions (Invitrogen) and was further purified using RNeasy columns (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Seven µg of total RNA were subjected to One-cycle Target Labeling Assay (Affymetrix) to generate biotinylated cRNA targets for hybridization to Affymetrix U133A 2.0 microarray
|
| |
|
| Hybridization protocol |
Standard hybridization to Affymetrix U133A 2.0 microarray
|
| Scan protocol |
Standard scanning protocol using GeneChip Scanner
|
| Description |
MCF-7 cells_NAMPT knockdown_replicate 1
|
| Data processing |
The raw data were processed by Affymetrix GCOS software to obtain detection calls and signal values, and then normalized by scaling. Signals were then log2 transformed, median centered for the array, and median centered for each gene.
|
| |
|
| Submission date |
Nov 03, 2008 |
| Last update date |
May 28, 2009 |
| Contact name |
W. Lee Kraus |
| E-mail(s) |
lee.kraus@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8511 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE13449 |
Expression analysis upon NAMPT knockdown in MCF-7 cells |
| GSE13577 |
SIRT1-Dependent Gene Regulation Through Promoter-Directed Recruitment of a Nuclear NAD+ Synthase |
|
